LOCUS       sequence-67420-e       10228 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for plasmid integration using the Flp-In system. AID at N-terminal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10228)
  AUTHORS   Holland AJ, Fachinetti D, Han JS, Cleveland DW
  TITLE     Inducible, reversible system for the rapid and complete degradation 
            of proteins in mammalian cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Dec 4;109(49):E3350-7. doi: 
            10.1073/pnas.1216880109. Epub 2012 Nov 12.
  PUBMED    23150568
REFERENCE   2  (bases 1 to 10228)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1216880109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-12-4- 4"; volume: "109"; issue: "49"; pages: "E3350-7"
COMMENT     Sequence Label: pcDNA5-EGFP-AID-BubR1
FEATURES             Location/Qualifiers
     source          1..10228
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1154..1183
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(1187..1189,1190..1900)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(1187..1189,1190..1900)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1229..1250)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1490..1509)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1837..1858
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1907..2590
                     /codon_start=1
                     /gene="Arabidopsis thaliana AXR3"
                     /product="auxin-responsive IAA17 protein"
                     /label=auxin-inducible degron
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="MGSVELNLRETELCLGLPGGDTVAPVTGNKRGFSETVDLKLNLNN
                     EPANKEGSTTHDVVTFDSKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSG
                     GPEAAAFVKVSMDGAPYLRKIDLRMYKSYDELSNALSNMFSSFTMGKHGGEEGMIDFMN
                     ERKLMDLVNSWDYVPSYEDKDGDWMLVGDVPWPMFVDTCKRLRLMKGSDAIGLAPRAME
                     KCKSRA"
     primer_bind     complement(6180..6197)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6186..6410
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6543..6562)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6694..6741
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6749..7771
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             6749..7771
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7901..8022
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7938..7957)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7992..8011
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8071..8087)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8071..8087)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8084..8106)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8095..8111
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8119..8125,8126..8143,8144..8149))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8164..8185
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8302..8319)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8473..9061)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8553..8572)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9232..10023,10024..10092))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9855..9874
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10093..10197)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccc cggggaattc ggcagatccc gaggtccgac
     1021 agcgcccggc ccagatcccc acgcctgcca ggagcaagcc gagagccagc cggccggcgc
     1081 actccgactc cgagcagtct ctgtccttcg acccgagccc cgcgcccttt ccgggacccc
     1141 tgccccgcgg atggagcaga agctcatctc agaagaagac ctcgacgtga gcaagggcga
     1201 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     1261 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     1321 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     1381 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     1441 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     1501 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     1561 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     1621 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     1681 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     1741 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     1801 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     1861 cgccgccggg atcactctcg gcatggacga gctgtacaag gcggatatgg gcagtgtcga
     1921 gctgaatctg agggagactg agctgtgtct tggtcttccc ggtggagata cagtggctcc
     1981 ggtaaccgga aacaagagag ggttctcaga gacggttgat ctgaagctaa atctgaataa
     2041 tgagcctgca aacaaggaag gatctacgac tcatgacgtc gtgacttttg attccaagga
     2101 gaagagtgct tgtcctaaag atccagccaa acctccggcc aaggcacaag ttgtgggatg
     2161 gccaccggtg agatcatacc ggaagaacgt gatggtttcc tgccaaaaat caagcggtgg
     2221 cccggaggcg gcggcgttcg tgaaggtatc aatggacgga gcaccgtact tgaggaaaat
     2281 cgatttgagg atgtataaaa gctacgatga gctttctaat gctttgtcca acatgttcag
     2341 ctcttttacc atgggcaaac atggaggaga agaaggaatg atagacttca tgaatgagag
     2401 gaaattgatg gatttggtga atagctggga ctatgttccc tcttatgaag acaaagacgg
     2461 tgattggatg ctcgtcggcg acgttccttg gccaatgttc gtcgatacat gcaagcgttt
     2521 acgtctcatg aaaggatcgg atgccattgg tctcgctccg agggcgatgg agaagtgcaa
     2581 gagcagagct ggatcccata tggcggcggt gaagaaggaa gggggtgctc tgagtgaagc
     2641 catgtccctg gagggagatg aatgggaact gagtaaagaa aatgtacaac ctttaaggca
     2701 agggcggatc atgtccacgc ttcagggagc actggcacaa gaatctgcct gtaacaatac
     2761 tcttcagcag cagaaacggg catttgaata tgaaattcga ttttacactg gaaatgaccc
     2821 tctggatgtt tgggataggt atatcagctg gacagagcag aactatcctc aaggtgggaa
     2881 ggagagtaat atgtcaacgt tattagaaag agctgtagaa gcactacaag gagaaaaacg
     2941 atattatagt gatcctcgat ttctcaatct ctggcttaaa ttagggcgtt tatgcaatga
     3001 gcctttggat atgtacagtt acttgcacaa ccaagggatt ggtgtttcac ttgctcagtt
     3061 ctatatctca tgggcagaag aatatgaagc tagagaaaac tttaggaaag cagatgcgat
     3121 atttcaggaa gggattcaac agaaggctga accactagaa agactacagt cccagcaccg
     3181 acaattccaa gctcgagtgt ctcggcaaac tctgttggca cttgagaaag aagaagagga
     3241 ggaagttttt gagtcttctg taccacaacg aagcacacta gctgaactaa agagcaaagg
     3301 gaaaaagaca gcaagagctc caatcatccg tgtaggaggt gctctcaagg ctccaagcca
     3361 gaacagagga ctccaaaatc catttcctca acagatgcaa aataatagta gaattactgt
     3421 ttttgatgaa aatgctgatg aggcttctac agcagagttg tctaagccta cagtccagcc
     3481 atggatagca ccccccatgc ccagggccaa agagaatgag ctgcaagcag gcccttggaa
     3541 cacaggcagg tccttggaac acaggcctcg tggcaataca gcttcactga tagctgtacc
     3601 cgctgtgctt cccagtttca ctccatatgt ggaagagact gcacaacagc cagttatgac
     3661 accatgtaaa attgaaccta gtataaacca catcctaagc accagaaagc ctggaaagga
     3721 agaaggagat tctctacaaa gggttcagag ccatcagcaa gcgtctgagg agaagaaaga
     3781 gaagatgatg tattgtaagg agaagattta tgcaggagta ggggaattct cctttgaaga
     3841 aattcgggct gaagttttcc ggaagaaatt aaaagagcaa agggaagccg agctattgac
     3901 cagtgcagag aagagagcag aaatgcagaa acagattgaa gagatggaga agaagctaaa
     3961 agaaatccaa actactcagc aagaaagaac aggtgatcag caagaagaga cgatgcctac
     4021 aaaggagaca actaaactgc aaattgcttc cgagtctcag aaaataccag gaatgactct
     4081 atccagttct gtttgtcaag taaactgttg tgccagagaa acttcacttg cggagaacat
     4141 ttggcaggaa caacctcatt ctaaaggtcc cagtgtacct ttctccattt ttgatgagtt
     4201 tcttctttca gaaaagaaga ataaaagtcc tcctgcagat cccccacgag ttttagctca
     4261 acgaagaccc cttgcagttc tcaaaacctc agaaagcatc acctcaaatg aagatgtgtc
     4321 tccagatgtt tgtgatgaat ttacaggaat tgaacccttg agcgaggatg ccattatcac
     4381 aggcttcaga aatgtaacaa tttgtcctaa cccagaagac acttgtgact ttgccagagc
     4441 agctcgtttt gtatccactc cttttcatga gataatgtcc ttgaaggatc tcccttctga
     4501 tcctgagaga ctgttaccgg aagaagatct agatgtaaag acctctgagg accagcagac
     4561 agcttgtggc actatctaca gtcagactct cagcatcaag aagctgagcc caattattga
     4621 agacagtcgt gaagccacac actcctctgg cttctctggt tcttctgcct cggttgcaag
     4681 cacctcctcc atcaaatgtc ttcaaattcc tgagaaacta gaacttacta atgagacttc
     4741 agaaaaccct actcagtcac catggtgttc acagtatcgc agacagctac tgaagtccct
     4801 accagagtta agtgcctctg cagagttgtg tatagaagac agaccaatgc ctaagttgga
     4861 aattgagaag gaaattgaat taggtaatga ggattactgc attaaacgag aatacctaat
     4921 atgtgaagat tacaagttat tctgggtggc gccaagaaac tctgcagaat taacagtaat
     4981 aaaggtatct tctcaacctg tcccatggga cttttatatc aacctcaagt taaaggaacg
     5041 tttaaatgaa gattttgatc atttttgcag ctgttatcaa tatcaagatg gctgtattgt
     5101 ttggcaccaa tatataaact gcttcaccct tcaggatctt ctccaacaca gtgaatatat
     5161 tacccatgaa ataacagtgt tgattattta taaccttttg acaatagtgg agatgctaca
     5221 caaagcagaa atagtccatg gtgacttgag tccaaggtgt ctgattctca gaaacagaat
     5281 ccacgatccc tatgattgta acaagaacaa tcaagctttg aagatagtgg acttttccta
     5341 cagtgttgac cttagggtgc agctggatgt ttttaccctc agcggctttc ggactgtaca
     5401 gatcctggaa ggacaaaaga tcctggctaa ctgttcttct ccctaccagg tagacctgtt
     5461 tggtatagca gatttagcac atttactatt gttcaaggaa cacctacagg tcttctggga
     5521 tgggtccttc tggaaactta gccaaaatat ttctgagcta aaagatggtg aattgtggaa
     5581 taaattcttt gtgcggattc tgaatgccaa tgatgaggcc acagtgtctg ttcttgggga
     5641 gcttgcagca gaaatgaatg gggtttttga cactacattc caaagtcacc tgaacaaagc
     5701 cttatggaag gtagggaagt taactagtcc tggggctttg ctctttcagt gagctaggca
     5761 atcaagtctc acagattgct gcctcagagc aatggttgta ttgtggaaca ctgaaactgt
     5821 atgtgctgta atttaattta ggacacattt agatgcacta ccattgctgt tctacttttt
     5881 ggtacaggta tattttgacg tcactgatat tttttataca gtgatatact tactcatggc
     5941 cttgtctaac ttttgtgaag aactatttta ttctaaacag actcattaca aatggttacc
     6001 ttgttattta acccatttgt ctctactttt ccctgtactt ttcccatttg taatttgtaa
     6061 aatgttctct tatgatcacc atgtattttg taaataataa aatagtatct gttaaaaaaa
     6121 aaaaaaaaaa aaaaaaaaaa actcgagcgg ccgcgggccc gtttaaaccc gctgatcagc
     6181 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     6241 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     6301 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     6361 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     6421 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     6481 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     6541 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     6601 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     6661 aaaacttgat tagggtgatg gttcacgtac ctagaagttc ctattccgaa gttcctattc
     6721 tctagaaagt ataggaactt ccttggccaa aaagcctgaa ctcaccgcga cgtctgtcga
     6781 gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga
     6841 agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag
     6901 ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct
     6961 cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc
     7021 ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct
     7081 gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg
     7141 gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg
     7201 cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc
     7261 gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg
     7321 gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac
     7381 agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat
     7441 cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag
     7501 gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga
     7561 ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg
     7621 atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag
     7681 aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg
     7741 ccccagcact cgtccgaggg caaaggaata gcacgtacta cgagatttcg attccaccgc
     7801 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     7861 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     7921 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     7981 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     8041 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     8101 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     8161 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     8221 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     8281 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     8341 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     8401 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8461 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8521 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8581 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8641 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8701 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8761 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8821 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8881 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     8941 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     9001 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     9061 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     9121 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     9181 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     9241 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     9301 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9361 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9421 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9481 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9541 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9601 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9661 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9721 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9781 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9841 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9901 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9961 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    10021 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    10081 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    10141 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    10201 atttccccga aaagtgccac ctgacgtc
//
