LOCUS       sequence-67423-e         723 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a Gateway-compatible retroviral destination vector which adds a 
            myristoylation sequence and a FLAG tag to each introduced ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene (Kinase Library Clones)
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pWZL-Neo-Myr-Flag-DEST
FEATURES             Location/Qualifiers
     source          1..723
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             376..681
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     560..579
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 acagtactgc gatgagtggc agggcggggc gtaaagatct ggatccggct tactaaaagc
       61 cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat
      121 gtatacccga agtatgtcaa aaagaggtat gctatgaagc agcgtattac agtgacagtt
      181 gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa
      241 gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc
      301 aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga
      361 acaggggctg gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt
      421 ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc
      481 ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat
      541 atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt
      601 atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac
      661 ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga
      721 cca
//
