LOCUS       sequence-67431-e         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Modified pcDNA3.1 vector for ligation independent cloning, including
            a ccdB cassette for eliminating no-insert background.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  TITLE     pUB2.1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUB2.1
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(97..116)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(98..116)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(161..364)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(190..210)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        365..744
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
       61 aatctcgact gtttaaacgc tagccagctt gggtctccct atagtgagtc gtattaattt
      121 cgataagcca gtaagcagtg ggttctctag ttagccagag agctctgctt atatagacct
      181 cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta cgacattttg
      241 gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg gggtggagac
      301 ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc caaaaccgca
      361 tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga aagtcccata
      421 aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg
      481 gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac cgtaaatact
      541 ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca tacgtcatta
      601 ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt accgtaagtt
      661 atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt gattactatt
      721 aataactagt caataatcaa tgtcaacgcg tatatctggc ccgtacatcg cgaagcagcg
      781 caaaacgcct aaccctaagc aga
//
