LOCUS       sequence-67441-e         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Hirai H, Tani T, Katoku-Kikyo N, Kellner S, Karian P, Firpo M, Kikyo
            N
  TITLE     Radical acceleration of nuclear reprogramming by chromatin 
            remodeling with the transactivation domain of MyoD.
  JOURNAL   Stem Cells. 2011 Sep;29(9):1349-61. doi: 10.1002/stem.684.
  PUBMED    21732495
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/stem"; 
            journalName: "Stem Cells"; date: "2011-09"; volume: "29"; issue: 
            "9"; pages: "1349-61"
COMMENT     Sequence Label: pMXs-hM₃O-IP
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             150..752
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MVTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     649..669
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
       61 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
      121 aaaaacacga tgataatatg gccacaacca tggttaccga gtacaagccc acggtgcgcc
      181 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
      241 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
      301 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
      361 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
      421 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
      481 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
      541 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
      601 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
      661 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
      721 cctggtgcat gacccgcaag cccggtgcct gagtcgacga taaaataaaa gattttattt
      781 agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa
      841 gtaacgccat tttgcaaggc atggaaaaat acataactga gaatagagaa gttcagatca
      901 aggtcaggaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      961 tcc
//
