LOCUS       sequence-67442-e         733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Addis RC, Ifkovits JL, Pinto F, Kellam LD, Esteso P, Rentschler S, 
            Christoforou N, Epstein JA, Gearhart JD
  TITLE     Optimization of direct fibroblast reprogramming to cardiomyocytes 
            using calcium activity as a functional measure of success.
  JOURNAL   J Mol Cell Cardiol. 2013 Apr 13;60C:97-106. doi: 
            10.1016/j.yjmcc.2013.04.004.
  PUBMED    23591016
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Cell 
            Cardiol."; date: "2013-04-13"; volume: "60C:97-106. doi"; pages: " 
            10.1016/j.yjmcc.2013.04.004"
COMMENT     Sequence Label: tetO-GATA4
FEATURES             Location/Qualifiers
     source          1..733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     60..79
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        60..78
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    110..134
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gctgttttga cctccataga agacaccggg accgatccag cctccgcggc cccgaattgt
       61 aatacgactc actataggga gacccaagct ggctaggtaa gcttgatcaa caagtttgta
      121 caaaaaagca ggctcgggag ctcgcaggga ccatgtatca gagcttggcc atggccgcca
      181 accacgggcc gccccccggt gcctacgagg cgggcggccc cggcgccttc atgcacggcg
      241 cgggcgccgc gtcctcgcca gtctacgtgc ccacaccgca ggtgccctcc tccgtgctga
      301 gcctgtccta cctccagggc ggaggcgcgg gctctgcgtc cggaggcgcc tcgggcggca
      361 gctccggtgg ggccgcgtct ggtgcggggc ccgggaccca gcagggcagc ccgggatgga
      421 gccaggcggg agccgacgga gccgcttaca ccccgccgcc ggtgtcgccg cgcttctcct
      481 tcccggggac caccgggtcc ctggcggccg ccgccgccgc tgccgcggcc cgggaagctg
      541 cggcctacag cagtggcggc ggagcggcgg gtgcgggcct ggcgggccgc gagcagtacg
      601 ggcgcgccgg cttcgcgggc tcctactcca gcccctaccc ggcttacatg gccgacgtgg
      661 gcgcgtcctg ggccgcagcc gccgccgcct ccgccggccc cttcgacagc ccggtcctgc
      721 acagcctgcc cgg
//
