LOCUS       sequence-67444-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Addis RC, Ifkovits JL, Pinto F, Kellam LD, Esteso P, Rentschler S, 
            Christoforou N, Epstein JA, Gearhart JD
  TITLE     Optimization of direct fibroblast reprogramming to cardiomyocytes 
            using calcium activity as a functional measure of success.
  JOURNAL   J Mol Cell Cardiol. 2013 Apr 13;60C:97-106. doi: 
            10.1016/j.yjmcc.2013.04.004.
  PUBMED    23591016
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Cell 
            Cardiol."; date: "2013-04-13"; volume: "60C:97-106. doi"; pages: " 
            10.1016/j.yjmcc.2013.04.004"
COMMENT     Sequence Label: TroponinT-Gateway
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(146..151,152..169,170..176)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(296..315)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt
       61 agattttgca taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat
      121 ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg
      181 gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa tggagaaaaa
      241 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc
      301 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt
      361 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc
      421 ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat
      481 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc
      541 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt
      601 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt
      661 cgtttcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga
      721 caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct
      781 gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat
      841 gcttaatgaa ttacaacagt actgcgatga
//
