LOCUS       sequence-67445-e        1018 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Shen MW, Fang F, Sandmeyer S, Da Silva NA
  TITLE     Development and characterization of a vector set with regulated 
            promoters for systematic metabolic engineering in Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2012 Dec;29(12):495-503. doi: 10.1002/yea.2930. Epub 2012 Nov
            19.
  PUBMED    23166051
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.2930"; 
            journalName: "Yeast"; date: "2012-12"; volume: "29"; issue: "12"; 
            pages: "495-503"
COMMENT     Sequence Label: pXP722
FEATURES             Location/Qualifiers
     source          1..1018
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(69..102)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(631..647)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(631..647)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(644..666)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    655..671
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(679..685,686..703,704..709))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    724..745
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(862..879)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tataagtaaa tgcatgtata ctaaactcac aaattagagc ttcaatttaa ttatatcagt
       61 tattacccat aacttcgtat agcatacatt atacgaagtt atcccgggta ccgagctcga
      121 attcgtaact tacacgcgcc tcgtatcttt taatgatgga ataatttggg aatttactct
      181 gtgtttattt atttttatgt tttgtatttg gattttagaa agtaaataaa gaaggtagaa
      241 gagttacgga atgaagaaaa aaaaataaac aaaggtttaa aaaatttcaa caaaaagcgt
      301 actttacata tatatttatt agacaagaaa agcagattaa atagatatac attcgattaa
      361 cgataagtaa aatgtaaaat cacaggattt tcgtgtgtgg tcttctacac agacaagatg
      421 aaacaattcg gcattaatac ctgagagcag gaagagcaag ataaaaggta gtatttgttg
      481 gcgatccccc tagagtcttt tacatcttcg gaaaacaaaa actatttttt ctttaatttc
      541 tttttttact ttctattttt aatttatata tttatattaa aaaatttaaa ttataattat
      601 ttttatagca cgtgatgaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta
      661 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
      721 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
      781 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
      841 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
      901 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
      961 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggcc
//
