LOCUS       sequence-67448-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Cohen DM, Chen H, Johnson RP, Choudhury B, Craig SW
  TITLE     Two distinct head-tail interfaces cooperate to suppress activation 
            of vinculin by talin.
  JOURNAL   J Biol Chem. 2005 Apr 29;280(17):17109-17. Epub 2005 Feb 22.
  PUBMED    15728584
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2005-04-29"; volume: "280(17)"; pages: "17109-17. 
            Epub 2005 Feb 22"
COMMENT     Sequence Label: pET15b/GgVcl V1-258 (aka VD1)
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(815..832)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(842..859)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             878..900
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatcctcga gctaccaggc atcttcatcc caggaagtga
       61 gttgtaatac acggattatt tcatttatct cagcactcat tttctctact gtgaaattgc
      121 gatttttcaa ggcctcttct attccctggc ttttagtgtt ttttgtggtt acaaagatct
      181 tcatagctga aataagtaca ggcaatagct ccttcacagt attcatggag ttcaccagca
      241 taaccctatg ttcctgatga gttaattcct gttgtctctc atcgatcatt ttcgccatct
      301 ttgtcattcc tggccctaga ttctttgtat atgtcaccaa atcctccata gtctctacta
      361 cttctgccac agtcagatat tccaatattc ctttgcagac acggatgatt ttacggacct
      421 ccgcttcatc aaatgtcaga agtaagtctg atgttccaga aaggatgcct cttgatccat
      481 caattaggta gtcacgagct ggtactgaat aaggatctgc ttgcagcatc tgggctgctc
      541 gaacgagctt ggtgcaggca ttctctactt tgatgaatgc tggtggcata tcccttttca
      601 agatctggtc ttctgttgtc tgcacagttt cttttccaac ccgcaccagg ttgctgacag
      661 cggcctgcac ggccgacacg ggggcggtga ggtccgggat ggccttgccg tctacctccc
      721 cctcctcgtg catgatgacc aggtgggaga tctgctgagc cacgggctcc aagatgctct
      781 cgatggtgcg cgtgtggaag acgggcatca tatggctgcc gcgcggcacc aggccgctgc
      841 tgtgatgatg atgatgatgg ctgctgccca tggtatatct ccttcttaaa gttaaacaaa
      901 attatt
//
