LOCUS       sequence-67451-e        1641 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ads under Pgrac promoter (inducible by IPTG) in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Zhou K, Zou R, Zhang C, Stephanopoulos G, Too HP
  TITLE     Optimization of amorphadiene synthesis in Bacillus subtilis via 
            transcriptional, translational, and media modulation.
  JOURNAL   Biotechnol Bioeng. 2013 Mar 8. doi: 10.1002/bit.24900.
  PUBMED    23483530
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2013 Mar 8. doi: 10.1002/bit.24900."
COMMENT     Sequence Label: pHT-ads
FEATURES             Location/Qualifiers
     source          1..1641
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgtctctga ctgaggaaaa accaatccgt ccgatcgcaa actttccgcc gagcatctgg
       61 ggtgatcagt tcctgatcta ccagaagcag gtcgagcaag gcgtagaaca gatcgtgaac
      121 gacctgaaaa aggaggtacg tcagctgctg aaagaggctc tggacatccc gatgaaacat
      181 gcgaacctgc tgaagctgat tgatgagatt cagcgcctgg gtatcccata ccacttcgaa
      241 cgtgaaattg atcacgcgct gcagtgtatt tatgaaacgt atggtgataa ctggaacggt
      301 gaccgtagct ccctgtggtt ccgtctgatg cgtaagcagg gttattacgt gacctgcgac
      361 gtcttcaaca actacaaaga caagaacggc gcgtttaaac agagcctggc gaatgacgtt
      421 gaaggcctgc tggagctgta tgaagcaacc tctatgcgtg ttccgggcga aatcatcctg
      481 gaagatgctc tgggtttcac ccgttctcgc ctgtctatca tgaccaagga cgcattttcc
      541 actaacccgg ccctgttcac cgaaatccag cgtgcgctga aacagcctct gtggaagcgt
      601 ctgccgcgta tcgaggcggc acagtacatc ccgttctatc agcaacagga ttcccacaac
      661 aaaaccctgc tgaaactggc gaaactggaa tttaacctgc tgcaatccct gcacaaagaa
      721 gaactgtctc atgtttgcaa atggtggaaa gccttcgaca tcaagaaaaa cgcgccgtgc
      781 ctgcgcgacc gtatcgtaga atgctatttc tggggcctgg gctccggtta tgagcctcaa
      841 tactctcgtg ctcgcgtatt cttcaccaaa gcggtagctg tgatcaccct gatcgacgat
      901 acctacgatg cttacggtac gtacgaagaa ctgaagattt ttaccgaggc tgttgaacgc
      961 tggtctatca cttgcctgga taccctgccg gaatacatga aaccgatcta taaactgttc
     1021 atggatactt ataccgaaat ggaagagttc ctggcaaaag aaggtcgcac tgatctgttc
     1081 aactgtggca aggagtttgt gaaagaattc gtccgcaacc tgatggtgga ggcgaagtgg
     1141 gcaaacgagg gtcacatccc gaccaccgaa gaacacgacc cggtagttat catcaccggt
     1201 ggcgcaaacc tgctgactac cacttgctac ctgggtatgt ccgacatttt tacgaaagaa
     1261 agcgttgaat gggcagtgtc tgcgcctccg ctgttccgtt actctggtat cctgggccgt
     1321 cgcctgaacg atctgatgac ccataaagcc gagcaggaac gtaaacactc cagcagctct
     1381 ctggaatctt acatgaaaga atacaacgtt aacgaagagt atgcgcagac cctgatctac
     1441 aaagaggttg aggatgtatg gaaagacatc aaccgtgagt acctgactac taaaaacatc
     1501 ccacgcccgc tgctgatggc agtgatttat ctgtgccagt ttctggaagt gcagtatgcg
     1561 ggcaaagata acttcacccg tatgggtgac gaatacaaac acctgatcaa gtccctgctg
     1621 gtttacccaa tgtccatctg a
//
