LOCUS       sequence-67455-e         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For in vivo expression of Cas9 or generation of Cas9 mRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Gagnon JA, Valen E, Thyme SB, Huang P, Ahkmetova L, Pauli A, 
            Montague TG, Zimmerman S, Richter C, Schier AF
  TITLE     Efficient mutagenesis by Cas9 protein-mediated oligonucleotide 
            insertion and large-scale assessment of single-guide RNAs.
  JOURNAL   PLoS One. 2014 May 29;9(5):e98186. doi: 
            10.1371/journal.pone.0098186. eCollection 2014.
  PUBMED    24873830
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-29"; volume: "9(5):e98186. doi"; pages: " 
            10.1371/journal.pone.0098186. eCollection 2014"
COMMENT     Sequence Label: pCS2-Cas9
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tnttttgngg atccatcgat gccaccatgg acaagaagta ctccattggg ctcgatatcg
       61 gcacaaacag cgtcggctgg gccgtcatta cggacgagta caaggtgccg agcaaaaaat
      121 tcaaagttct gggcaatacc gatcgccaca gcataaagaa gaacctcatt ggcgccctcc
      181 tgttcgactc cggggagacg gccgaagcca cgcggctcaa aagaacagca cggcgcagat
      241 atacccgcag aaagaatcgg atctgctacc tgcaggagat ctttagtaat gagatggcta
      301 aggtggatga ctctttcttc cataggctgg aggagtcctt tttggtggag gaggataaaa
      361 agcacgagcg ccacccaatc tttggcaata tcgtggacga ggtggcgtac catgaaaagt
      421 acccaaccat atatcatctg aggaagaagc ttgtagacag tactgataag gctgacttgc
      481 ggttgatcta tctcgcgctg gcgcatatga tcaaatttcg gggacacttc ctcatcgagg
      541 gggacctgaa cccagacaac agcgatgtcg acaaactctt tatccaactg gttcagactt
      601 acaatcagct tttcgaagag aacccgatca acgcatccgg agttgacgcc aaagcaatcc
      661 tgagcgctag gctgtccaaa tcccggcggc tcgaaaacct catcgcacag ctccctgggg
      721 agaagaagaa cggcctgttt ggtaatctta tcgccctgtc actcgggctg acccccaact
      781 ttaaatctaa ct
//
