LOCUS       sequence-67458-e        1348 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For in vitro expression  and purification of Cas9 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Gagnon JA, Valen E, Thyme SB, Huang P, Ahkmetova L, Pauli A, 
            Montague TG, Zimmerman S, Richter C, Schier AF
  TITLE     Efficient mutagenesis by Cas9 protein-mediated oligonucleotide 
            insertion and large-scale assessment of single-guide RNAs.
  JOURNAL   PLoS One. 2014 May 29;9(5):e98186. doi: 
            10.1371/journal.pone.0098186. eCollection 2014.
  PUBMED    24873830
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-29"; volume: "9(5):e98186. doi"; pages: " 
            10.1371/journal.pone.0098186. eCollection 2014"
COMMENT     Sequence Label: pET-28b-Cas9-His
FEATURES             Location/Qualifiers
     source          1..1348
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gggggaattc ctcagaatat tttgttaact ttagaaggag atataccatg gacaagaagt
       61 actccattgg gctcgatatc ggcacaaaca gcgtcggctg ggccgtcatt acggacgagt
      121 acaaggtgcc gagcaaaaaa ttcaaagttc tgggcaatac cgatcgccac agcataaaga
      181 agaacctcat tggcgccctc ctgttcgact ccggggagac ggccgaagcc acgcggctca
      241 aaagaacagc acggcgcaga tatacccgca gaaagaatcg gatctgctac ctgcaggaga
      301 tctttagtaa tgagatggct aaggtggatg actctttctt ccataggctg gaggagtcct
      361 ttttggtgga ggaggataaa aagcacgagc gccacccaat ctttggcaat atcgtggacg
      421 aggtggcgta ccatgaaaag tacccaacca tatatcatct gaggaagaag cttgtagaca
      481 gtactgataa ggctgacttg cggttgatct atctcgcgct ggcgcatatg atcaaatttc
      541 ggggacactt cctcatcgag ggggacctga acccagacaa cagcgatgtc gacaaactct
      601 ttatccaact ggttcagact tacaatcagc ttttcgaaga gaacccgatc aacgcatccg
      661 gagttgacgc caaagcaatc ctgagcgcta ggctgtccaa atcccggcgg ctcgaaaacc
      721 tcatcgcaca gctccctggg gagaagaaga acggcctgtt tggtaatctt atcgccctgt
      781 cactcgggct gacccccaac tttaaatcta acttcgacct ggccgaagat gccaagcttc
      841 aactgagcaa agacacctac gatgatgatc tcgacaatct gctggcccag atcggcgacc
      901 agtacgcaga cctttttttg gtcgcaaaga acctgtcaga cgccattctg ctgagtgata
      961 ttctgcgagt gaaacacgag atcaccaagc tccgctgagc gctagtatga tcaagcgcta
     1021 tgatgagcat caccnagact tgactttgct gaangccntt gtcaaacagc aacggcctga
     1081 agagtacaag gaattttcnt cgatcagtct aaaaaggcta cgccggatca tttgacgcgg
     1141 agcaagccag gagaattttt acaattttat agcccnatct gaaaaaatga acgaaccaag
     1201 aactgctggt aacctaacca aaaaattgtt gcccaaagng acctccgaca tggagcatcc
     1261 ccacaaatcc cgtgggacgt accttcctag cgggaaagaa ttcccccttt taaataacgg
     1321 gaaaaatgga aaaccctctt tgaaaccc
//
