LOCUS       sequence-67459-e        1297 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For in vitro expression  and purification of Cas9 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Gagnon JA, Valen E, Thyme SB, Huang P, Ahkmetova L, Pauli A, 
            Montague TG, Zimmerman S, Richter C, Schier AF
  TITLE     Efficient mutagenesis by Cas9 protein-mediated oligonucleotide 
            insertion and large-scale assessment of single-guide RNAs.
  JOURNAL   PLoS One. 2014 May 29;9(5):e98186. doi: 
            10.1371/journal.pone.0098186. eCollection 2014.
  PUBMED    24873830
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-29"; volume: "9(5):e98186. doi"; pages: " 
            10.1371/journal.pone.0098186. eCollection 2014"
COMMENT     Sequence Label: pET-28b-Cas9-His
FEATURES             Location/Qualifiers
     source          1..1297
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(38..55)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(71..91)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gggcatcact ctttgggctt tgtacagccg gatctcagtg gtggtggtgg tggtgctcga
       61 gtgcggccgc caccttcctc ttcttcttgg ggtcagccct gctgtctcca ccgagctgag
      121 agaggtcgat tcttgtttca tagagccccg taattgactg atgaatcagt gtggcgtcca
      181 ggacctcctt tgtagaggtg taccgctttc tgtctatggt ggtgtcgaag tacttgaagg
      241 ctgcaggcgc gcccaagttg gtcagagtaa acaagtggat aatgttttct gcctgctccc
      301 tgatgggctt atccctgtgc ttattgtaag cagaaagcac cttatcgagg ttagcgtcgg
      361 cgaggatcac tcttttggag aattcgctta tttgctcgat gatctcatca aggtagtgtt
      421 tgtgttgttc cacgaacagc tgcttctgct cattatcttc gggagaccct ttgagctttt
      481 catagtggct ggccagatac aagaaattaa cgtatttaga gggcagtgcc agctcgttac
      541 ctttctgcag ctcgcccgca ctagcgagca ttcgtttccg gccgttttca agctcaaaga
      601 gagagtactt gggaagctta atgatgaggt cttttttgac ctctttatat cctttcgcct
      661 cgagaaagtc gatggggttt ttttcgaagc ttgatcgctc catgattgtg atgcccagca
      721 gttccttgac gcttttgagt tttttagact tccctttctc cactttggcc acaaccagta
      781 cactgtaagc gactgtagga gaatcgaatc cgccgtattt cttggggtcc caatcttttt
      841 tgcgtgcgat cagcttgtcg ctgttccttt tcgggaggat actttccttg nagaagccct
      901 cggtctgtac ttcggtcttt ttaacgatgt tcaccctgcg catggacagg accttccgga
      961 ctgtcgcgaa atccctaccc ttgtcccaca cgaattctcc ttgttctccg tttgttcgat
     1021 aagtggtcgc tttcgattct ctccatggnc agtgtaatct cggtcttgaa aaatcataat
     1081 atgctgtaaa agagtacttt agcgtggctt gcctattcct gctcagactt gcgatcattt
     1141 tctaactcgn acatttaagt tctcgtacca aattcgatca gcctgggtaa tttttgaaag
     1201 ggcagggcta cactgcattc ggtagcatca tgccaggggg gaattttgat tccctccctt
     1261 aaaacggaat cctttggaat tggacccctt aacttcc
//
