LOCUS       sequence-67469-e         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Huang L, Jin J, Deighan P, Kiner E, McReynolds L, Lieberman J
  TITLE     Efficient and specific gene knockdown by small interfering RNAs 
            produced in bacteria.
  JOURNAL   Nat Biotechnol. 2013 Apr;31(4):350-6. doi: 10.1038/nbt.2537. Epub 
            2013 Mar 10.
  PUBMED    23475073
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.2537"; 
            journalName: "Nat Biotechnol"; date: "2013-04"; volume: "31"; issue:
            "4"; pages: "350-6"
COMMENT     Sequence Label: pGEX-4T-1-p19-T7
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..27
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             544..561
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     568..587
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        568..586
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(704..726)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 tcaatcggac tggttccgcg tggatccatg gaacgagcta tacaaggaaa cgacgctagg
       61 gaacaagcta acagtgaacg ttgggatgga ggatcaggag gtaccacttc tcccttcaaa
      121 cttcctgacg aaagtccgag ttggactgag tggcggctac ataacgatga gacgaattcg
      181 aatcaagata atccccttgg tttcaaggaa agctggggtt tcgggaaagt tgtatttaag
      241 agatatctca gatacgacag gacggaagct tcactgcaca gagtccttgg atcttggacg
      301 ggagattcgg ttaactatgc agcatctcga tttttcggtt tcgaccagat cggatgtacc
      361 tatagtattc ggtttcgagg agttagtatc accgtttctg gagggtcgcg aactcttcag
      421 catctctgtg agatggcaat tcggtctaag caagaactgc tacagcttgc cccaatcgaa
      481 gtggaaagta atgtatcaag aggatgccct gaaggtactc aaaccttcga aaaagaaagc
      541 gagcaccacc accaccacca ctgaatctaa tacgactcac tataggggag ctcatcctcg
      601 agtctagagg gcccgtttaa acccgctgat cagcgtcgac actgcggccg catcgtgact
      661 gactgacgat ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
      721 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
      781 gcgcgtcacc gggtgttggc gggtg
//
