LOCUS       sequence-67470-e         615 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Huang L, Jin J, Deighan P, Kiner E, McReynolds L, Lieberman J
  TITLE     Efficient and specific gene knockdown by small interfering RNAs 
            produced in bacteria.
  JOURNAL   Nat Biotechnol. 2013 Apr;31(4):350-6. doi: 10.1038/nbt.2537. Epub 
            2013 Mar 10.
  PUBMED    23475073
REFERENCE   2  (bases 1 to 615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.2537"; 
            journalName: "Nat Biotechnol"; date: "2013-04"; volume: "31"; issue:
            "4"; pages: "350-6"
COMMENT     Sequence Label: pGEX-4T-1-p19-T7-EGFP100
FEATURES             Location/Qualifiers
     source          1..615
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..26
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             543..560
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     567..586
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        567..585
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aatcggatct ggttccgcgt ggatccatgg aacgagctat acaaggaaac gacgctaggg
       61 aacaagctaa cagtgaacgt tgggatggag gatcaggagg taccacttct cccttcaaac
      121 ttcctgacga aagtccgagt tggactgagt ggcggctaca taacgatgag acgaattcga
      181 atcaagataa tccccttggt ttcaaggaaa gctggggttt cgggaaagtt gtatttaaga
      241 gatatctcag atacgacagg acggaagctt cactgcacag agtccttgga tcttggacgg
      301 gagattcggt taactatgca gcatctcgat ttttcggttt cgaccagatc ggatgtacct
      361 atagtattcg gtttcgagga gttagtatca ccgtttctgg agggtcgcga actcttcagc
      421 atctctgtga gatggcaatt cggtctaagc aagaactgct acagcttgcc ccaatcgaag
      481 tggaaagtaa tgtatcaaga ggatgccctg aaggtactca aaccttcgaa aaagaaagcg
      541 agcaccacca ccaccaccac tgaatctaat acgactcact ataggggagc tcccgctacc
      601 ccgaccacat gaagc
//
