LOCUS       sequence-67477-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Ellila S, Jurvansuu JM, Iwai H
  TITLE     Evaluation and comparison of protein splicing by exogenous inteins 
            with foreign exteins in Escherichia coli.
  JOURNAL   FEBS Lett. 2011 Oct 10.
  PUBMED    22001202
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2011 Oct 10."
COMMENT     Sequence Label: pMMDuet19
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..88
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 attttgttta actttaataa ggagatatac catgggcagc agccatcatc atcatcatca
       61 cagcagcggc ctggtgccgc gcggcagcct aggacagtac aaacttatcc tgaacggtaa
      121 aaccctgaaa ggtgaaacca ccaccgaagc tgttgacgct gctaccgcgg aaaaagtttt
      181 caaacagtac gctaacgaca acggtgttga cggtgaatgg acctacgacg acgctaccaa
      241 aacctacacc gtaacggaag gatccggttg tttagcaggc gatagtctgg tgacattagt
      301 agatagcgga ctgcaagtgc caattaagga attagtaggt aaatctggtt ttgcagtttg
      361 ggcattgaac gaagctacaa tgcagctaga aaaggcaatt gttagcaatg ctttttcgac
      421 tggtataaag cctctgttta ctttgacaac tcgattgggg cggaaaatcc gtgccacagg
      481 taatcacaag tttttaacaa ttaatggctg gaaaagactt gatgaattaa ctcctaaaga
      541 acatctcgct ttaccaagaa atctccccag ttctggtact cttgccaaaa gtgatattta
      601 ttgggatgaa atagtttcaa ttgaatatag tggtgaggaa gaagtgtttg acctcaccgt
      661 tcctggtttg cataattttg ttgcaaataa tattattgtt cacaattcca ttggtaccca
      721 gtacaaactt atcctgaacg gtaaaaccct gaaaggtgaa accaccaccg aagctgttga
      781 cgctgctacc gcggaaaaag ttttcaaaca
//
