LOCUS       sequence-67480-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Ellila S, Jurvansuu JM, Iwai H
  TITLE     Evaluation and comparison of protein splicing by exogenous inteins 
            with foreign exteins in Escherichia coli.
  JOURNAL   FEBS Lett. 2011 Oct 10.
  PUBMED    22001202
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2011 Oct 10."
COMMENT     Sequence Label: pSEDuet40
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttttgtttaa ctttaataag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcactagta aaatcgaaga aggtaaactg gtaatctgga ttaacggcga taaaggctat
      121 aacggtctcg ctgaagtcgg taagaaattc gagaaagata ccggaattaa agtcaccgtt
      181 gagcatccgg ataaactgga agagaaattc ccacaggttg cggcaactgg cgatggccct
      241 gacattatct tctgggcaca cgaccgcttt ggtggctacg ctcaatctgg cctgttggct
      301 gaaatcaccc cggacaaagc gttccaggac aagctgtatc cgtttacctg ggatgccgta
      361 cgttacaacg gcaagctgat tgcttacccg atcgctgttg aagcgttatc gctgatttat
      421 aacaaagatc tgctgccgaa cccgccaaaa acctgggaag agatcccggc gctggataaa
      481 gaactgaaag cgaaaggtaa gagcgcgctg atgttcaacc tgcaagaacc gtacttcacc
      541 tggccgctga ttgctgctga cgggggttat gcgttcaagt atgaaaacgg caagtacgac
      601 attaaagacg tgggcgtgga taacgctggc gcgaaagcgg gtctgacctt cctggttgac
      661 ctgattaaaa acaaacacat gaatgcagac accgattact ccatcgcaga agctgccttt
      721 aataaaggcg aaacagcgat gaccatcaac ggcccgtggg catggtccaa catcgacacc
      781 agcaaagtga attatggtgt aacggtactg ccgaccttca ag
//
