LOCUS       sequence-67481-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Ellila S, Jurvansuu JM, Iwai H
  TITLE     Evaluation and comparison of protein splicing by exogenous inteins 
            with foreign exteins in Escherichia coli.
  JOURNAL   FEBS Lett. 2011 Oct 10.
  PUBMED    22001202
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2011 Oct 10."
COMMENT     Sequence Label: pSEDuet40
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             749..793
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(831..849)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      845..892
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ccagtttacc ttgcggatgg caagacaata aaaataaagg atctatacag ttctgagaga
       61 aaaaaagaag ataacattgt tgaggctggt tcgggagaag agataataca tctaaaagat
      121 cccattcaaa tatattctta tgtggacggg accatagtca ggagcagatc aagacttcta
      181 tacaagggca agagctctta tcttgtgagg atagaaacta ttggcggaag atcggtaagc
      241 gttacaccag ttcacaaact ctttgtcctt acggaaaagg gtatcgaaga ggttatggcc
      301 tccaacctaa aggtaggcga catgattgct gctgtagcag aaagcgaatc tgaagcaaga
      361 gactgcggaa tgagcgagga atgcgtgatg gaagcagaag tttatacgtc acttgaagcg
      421 acattcgata gagtaaagtc tatagcgtac gagaagggtg attttgatgt atacgatctt
      481 tccgtaccag aatacggcag gaactttata ggcggagaag gacttctcgt acttcacaac
      541 acggttatag gtacccagta caaacttatc ctgaacggta aaaccctgaa aggtgaaacc
      601 accaccgaag ctgttgacgc tgctaccgcg gaaaaagttt tcaaacagta cgctaacgac
      661 aacggtgttg acggtgaatg gacctacgac gacgctacca aaacctacac cgtaacggaa
      721 taagcttgcg gccgcactcg agtctggtaa agaaaccgct gctgcgaaat ttgaacgcca
      781 gcacatggac tcgtctacta gcgcagctta attaacctag gctgctgcca ccgctgagca
      841 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaa
//
