LOCUS       sequence-67482-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Heinamaki K, Oeemig JS, Paakkonen K, Djupsjobacka J, Iwai H
  TITLE     NMR resonance assignment of DnaE intein from Nostoc punctiforme.
  JOURNAL   Biomol NMR Assign. 2009 Jun;3(1):41-3. doi: 
            10.1007/s12104-008-9137-1. Epub 2009 Jan 13.
  PUBMED    19636943
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12104-008-9137-1"; journalName: "Biomol NMR Assign"; date:
            "2009-06"; volume: "3"; issue: "1"; pages: "41-3"
COMMENT     Sequence Label: pJDJRSF05
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(13..57)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(506..796)
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIETHREQIGG"
ORIGIN
        1 tgcgctagta gacgagtcca tgtgctggcg ttcaaatttc gcagcagcgg tttctttacc
       61 agactcgagt gcggccgcaa gcttaattag aagctatgaa gccatttttg agtgcaaaat
      121 tatggtcgcg ctcaactcca atgtcataga cattttgttt gcctaaatat ttacgtgtgg
      181 ctattttgat attcggcaaa ttatcaaccc gcatcaaatc caattcacgt tcaaatattt
      241 catcaattgg caacatttga ccatcaacag tcataaactt atggtctttt gttgcccgaa
      301 tcaatgaacc atcttccaaa caatactcaa acacctcttg ttctccgcga tcgtgccatt
      361 gtgctacagg ttgtgtataa atatttccat tattatcaac gctataaaca gtacattcga
      421 tgcgcttttc tacaatttta ccaatcggta ataatccata ttctactgtc aatatttccg
      481 tttcatagct tagagctcct ccggatccac caatctgttc tctgtgagtc tcaataatat
      541 cgttatcctc catgtccaaa tcttcagggg tctgatcagc ttgaattcta ataccgtcgt
      601 acaagaatct taaggagtcc atttccttac cctgtctttt agcgaacgct tccatcagcc
      661 ttcttaaagg agtggtcttt ttgatcttaa agaagatttc tgaagatcca tcggacacct
      721 ttaaattgat gtgagtctca ggcttgactt ctggcttgac ctctggctta gcttcttgat
      781 tgacttctga gtcactagtg ctgtgatgat
//
