LOCUS       sequence-67483-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Heinamaki K, Oeemig JS, Paakkonen K, Djupsjobacka J, Iwai H
  TITLE     NMR resonance assignment of DnaE intein from Nostoc punctiforme.
  JOURNAL   Biomol NMR Assign. 2009 Jun;3(1):41-3. doi: 
            10.1007/s12104-008-9137-1. Epub 2009 Jan 13.
  PUBMED    19636943
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12104-008-9137-1"; journalName: "Biomol NMR Assign"; date:
            "2009-06"; volume: "3"; issue: "1"; pages: "41-3"
COMMENT     Sequence Label: pJDJRSF05
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..359
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIETHREQIGG"
     CDS             808..852
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 aattttgttt aactttaata aggagatata ccatgggcag cagccatcat catcatcatc
       61 acagcactag tgactcagaa gtcaatcaag aagctaagcc agaggtcaag ccagaagtca
      121 agcctgagac tcacatcaat ttaaaggtgt ccgatggatc ttcagaaatc ttctttaaga
      181 tcaaaaagac cactccttta agaaggctga tggaagcgtt cgctaaaaga cagggtaagg
      241 aaatggactc cttaagattc ttgtacgacg gtattagaat tcaagctgat cagacccctg
      301 aagatttgga catggaggat aacgatatta ttgagactca cagagaacag attggtggat
      361 ccggaggagc tctaagctat gaaacggaaa tattgacagt agaatatgga ttattaccga
      421 ttggtaaaat tgtagaaaag cgcatcgaat gtactgttta tagcgttgat aataatggaa
      481 atatttatac acaacctgta gcacaatggc acgatcgcgg agaacaagag gtgtttgagt
      541 attgtttgga agatggttca ttgattcggg caacaaaaga ccataagttt atgactgttg
      601 atggtcaaat gttgccaatt gatgaaatat ttgaacgtga attggatttg atgcgggttg
      661 ataatttgcc gaatatcaaa atagccacac gtaaatattt aggcaaacaa aatgtctatg
      721 acattggagt tgagcgcgac cataattttg cactcaaaaa tggcttcata gcttctaatt
      781 aagcttgcgg ccgcactcga gtctggtaaa gaaaccgctg ctgcgaaatt tgaacgccag
      841 cacatggact cgtctactag cgcagcttaa tta
//
