LOCUS       sequence-67485-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Carmine intensiometric genetically encoded Ca2+-indicators for 
            optical imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Wu J, Liu L, Matsuda T, Zhao Y, Rebane A, Drobizhev M, Chang YF, 
            Araki S, Arai Y, March K, Hughes TE, Sagou K, Miyata T, Nagai T, Li 
            WH, Campbell RE
  TITLE     Improved Orange and Red Ca Indicators and Photophysical 
            Considerations for Optogenetic Applications.
  JOURNAL   ACS Chem Neurosci. 2013 Mar 19.
  PUBMED    23452507
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Neurosci. 2013 Mar 19."
COMMENT     Sequence Label: CMV-CAR-GECO1
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(67..510)
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTFDFPEFLTMMARKMNDTDSEEEIREAFRVFDKDGNGYIGAAEL
                     RHVMTDLGEKLTDEEVDEMIRVADIDGDGQVNYEEFVQMMTAK"
     primer_bind     complement(534..553)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tatagaatag ggccctctag atgcatgctc gagcggccgc cagtgtgatg gatatctgca
       61 gaattcctac ttcgctgtca tcatttgtac aaactcttcg tagtttacct gaccatcccc
      121 atcgatgtct gctaccctga tcatttcatc aacctcctca tctgttaact tctctccaag
      181 gtctgtcatc acgtggcgaa gctctgctgc gccgatgtag ccattgccgt ccttatcaaa
      241 cacgcggaac gcttctctaa tttcctcttc actgtctgtg tcattcattt ttcttgccat
      301 catcgtcagg aactcaggga agtcgaatgt gccgtcaccg tcggcatcta cttcattgat
      361 catgtcctgc agctctgctt ctgtggggtt ctgccccaga gaccgcatca ccgtccccag
      421 ctccttggtt gttatcgtcc catccccgtc cttgtcaaat agggagaaag cctctttaaa
      481 ttctgcgatc tgctcttcag tcagttggtc acgcgtaggc tcccagccca tggtcttctt
      541 ctgcattacg gggccgtcgg gggggaagtt ggtgccgcgc agcttcacct tgtagatgaa
      601 tacgccgtcc tgcagggagg agtcctggtt aacgtgaata atgccgccgt cctcgaagtt
      661 catcacgcgc tcccacctga agccctcggg gaaggacagc ttgaagtagt cggggatgtc
      721 ggctgggtgc ttaatgtagg ccttggagcc gtacataatc tgaggggaca ggatgtccca
      781 ggcgaagggc agggggccac ccttggtcac cttc
//
