LOCUS       sequence-67488-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Red intensiometric genetically encoded Ca2+-indicators for optical 
            imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Wu J, Liu L, Matsuda T, Zhao Y, Rebane A, Drobizhev M, Chang YF, 
            Araki S, Arai Y, March K, Hughes TE, Sagou K, Miyata T, Nagai T, Li 
            WH, Campbell RE
  TITLE     Improved Orange and Red Ca Indicators and Photophysical 
            Considerations for Optogenetic Applications.
  JOURNAL   ACS Chem Neurosci. 2013 Mar 19.
  PUBMED    23452507
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Neurosci. 2013 Mar 19."
COMMENT     Sequence Label: CMV-mito-R-GECO1
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             112..186
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             208..282
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             331..387
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctggc tagcgtttaa acgggccctc tagactcgag catgtctgtt
      121 ctgactcctc tgctgctccg gggtctcaca ggttccgcaa gaagactccc cgtgcctagg
      181 gccaaaattc attcactggg ggaccccatg agcgtgctca ccccactcct gctgcggggg
      241 ctgaccggca gcgctaggcg gctgccagtc cccagggcca agatccacag tctcggcgat
      301 cccaaggatc caaccatggt cgactcttca cgtcgtaagt ggaataaggc aggtcacgca
      361 gtcagagcta taggtcggct gagctcaccc gtggtttccg agcggatgta ccccgaggac
      421 ggcgccctga agagcgagat caagaagggg ctgaggctga aggacggcgg ccactacgcc
      481 gccgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacatc
      541 gtcgacatca agttggacat cgtgtcccac aacgaggact acaccatcgt ggaacagtgc
      601 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gggaggtaca
      661 ggcgggagtc tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
      721 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
      781 ggcgagggcc gcccctacga ggcctttcag accgctaagc tgaaggtgac caagggtggc
      841 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc ca
//
