LOCUS       sequence-67490-e         663 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Carmine intensiometric genetically encoded Ca2+-indicators for 
            optical imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Wu J, Liu L, Matsuda T, Zhao Y, Rebane A, Drobizhev M, Chang YF, 
            Araki S, Arai Y, March K, Hughes TE, Sagou K, Miyata T, Nagai T, Li 
            WH, Campbell RE
  TITLE     Improved Orange and Red Ca Indicators and Photophysical 
            Considerations for Optogenetic Applications.
  JOURNAL   ACS Chem Neurosci. 2013 Mar 19.
  PUBMED    23452507
REFERENCE   2  (bases 1 to 663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Neurosci. 2013 Mar 19."
COMMENT     Sequence Label: CMV-mito-CAR-GECO1
FEATURES             Location/Qualifiers
     source          1..663
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..441)
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTFDFPEFLTMMARKMNDTDSEEEIREAFRVFDKDGNGYIGAAEL
                     RHVMTDLGEKLTDEEVDEMIRVADIDGDGQVNYEEFVQMMTAK"
     primer_bind     complement(465..484)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cttcgctgtc atcatttgta caaactcttc gtagtttacc tgaccatccc catcgatgtc
       61 tgctaccctg atcatttcat caacctcctc atctgttaac ttctctccaa ggtctgtcat
      121 cacgtggcga agctctgctg cgccgatgta gccattgccg tccttatcaa acacgcggaa
      181 cgcttctcta atttcctctt cactgtctgt gtcattcatt tttcttgcca tcatcgtcag
      241 gaactcaggg aagtcgaatg tgccgtcacc gtcggcatct acttcattga tcatgtcctg
      301 cagctctgct tctgtggggt tctgccccag agaccgcatc accgtcccca gctccttggt
      361 tgttatcgtc ccatccccgt ccttgtcaaa tagggagaaa gcctctttaa attctgcgat
      421 ctgctcttca gtcagttggt cacgcgtagg ctcccagccc atggtcttct tctgcattac
      481 ggggccgtcg ggggggaagt tggtgccgcg cagcttcacc ttgtagatga atacgccgtc
      541 ctgcagggag gagtcctggt taacgtgaat aatgccgccg tcctcgaagt tcatcacgcg
      601 ctcccacctg aagccctcgg ggaaggacag cttgaagtag tcggggatgt cggctgggtg
      661 ctt
//
