LOCUS       sequence-67491-e         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Carmine intensiometric genetically encoded Ca2+-indicators for 
            optical imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Wu J, Liu L, Matsuda T, Zhao Y, Rebane A, Drobizhev M, Chang YF, 
            Araki S, Arai Y, March K, Hughes TE, Sagou K, Miyata T, Nagai T, Li 
            WH, Campbell RE
  TITLE     Improved Orange and Red Ca Indicators and Photophysical 
            Considerations for Optogenetic Applications.
  JOURNAL   ACS Chem Neurosci. 2013 Mar 19.
  PUBMED    23452507
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Neurosci. 2013 Mar 19."
COMMENT     Sequence Label: CMV-mito-CAR-GECO1
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             117..191
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             213..287
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             336..392
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
ORIGIN
        1 cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag ctggctagcg tttaaacggg ccctctagac tcgagcatgt
      121 ctgttctgac tcctctgctg ctccggggtc tcacaggttc cgcaagaaga ctccccgtgc
      181 ctagggccaa aattcattca ctgggggacc ccatgagcgt gctcacccca ctcctgctgc
      241 gggggctgac cggcagcgct aggcggctgc cagtccccag ggccaagatc cacagtctcg
      301 gcgatcccaa ggatccaacc atggtcgact cttcacgtcg taagtggaat aaggcaggtc
      361 acgcagttag agctataggt cggctgagct cacccgtggt ttccgagcgg atgtaccccg
      421 aggacggcgc cctgaagagc gagatcaaga aggggctgag gctgaaggac ggcggccact
      481 acgccgccga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
      541 acatcgtcga catcaagttg gacatcgtgt cccacaacga ggactacacc atcgtggaac
      601 agtgcgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagggag
      661 gtacaggcgg gagtctggtg agcaagggcg tggaggataa catggccatc gtcaaggagt
      721 tcatgcgctt caagacgcac atagagggct ccgtgaacgg ccacgagttc
//
