LOCUS       sequence-67492-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Orange intensiometric genetically encoded Ca2+-indicators for 
            optical imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Wu J, Liu L, Matsuda T, Zhao Y, Rebane A, Drobizhev M, Chang YF, 
            Araki S, Arai Y, March K, Hughes TE, Sagou K, Miyata T, Nagai T, Li 
            WH, Campbell RE
  TITLE     Improved Orange and Red Ca Indicators and Photophysical 
            Considerations for Optogenetic Applications.
  JOURNAL   ACS Chem Neurosci. 2013 Mar 19.
  PUBMED    23452507
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Neurosci. 2013 Mar 19."
COMMENT     Sequence Label: CMV-O-GECO1
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             115..171
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWIKAGHAVRAIGRLSS"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagcttgg taccgagctc ggatccacca tggtcgactc atcacgtcgt
      121 aagtggatta aggcaggtca cgcagtcaga gctataggtc ggctgagctc acccgtggtt
      181 tccgagcgga tgtaccccga ggacggcgtc ctgaagagcg agatcaagaa ggggctgagg
      241 ctgaaggacg gcggccacta cgccgccgag gtcaagacca cctacaaggc caagaagccc
      301 gtgcagctgc ccggcgccta catcgtcgac ataaagttgg acatcgtgtc ccacaacgag
      361 gactacacca tcgtggaaca gtgcgaacgc gccgagggcc gccaccccac cggcggcagg
      421 gacgagctgt acaagggagg tacaggaggg agtctggtga gcaagggcga ggaggataac
      481 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
      541 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgaggcctt tcagaccgct
      601 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
      661 ttcacatacg gctccaaggc ctacattaag cacccagccg acatccccga ctactttaag
      721 ctgtccttcc ccgagggctt caggtgggag cgcgtgatga acttcgagga cggcggcatt
      781 attcacgtta accaggactc ctccctgcag gacggcgtat tcatctacaa ggtgaagctg
      841 cgcggcacca actt
//
