LOCUS       sequence-67499-e         603 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Huang L, Jin J, Deighan P, Kiner E, McReynolds L, Lieberman J
  TITLE     Efficient and specific gene knockdown by small interfering RNAs 
            produced in bacteria.
  JOURNAL   Nat Biotechnol. 2013 Apr;31(4):350-6. doi: 10.1038/nbt.2537. Epub 
            2013 Mar 10.
  PUBMED    23475073
REFERENCE   2  (bases 1 to 603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.2537"; 
            journalName: "Nat Biotechnol"; date: "2013-04"; volume: "31"; issue:
            "4"; pages: "350-6"
COMMENT     Sequence Label: pGEX-4T-1-p19-T7-GagB500
FEATURES             Location/Qualifiers
     source          1..603
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             8..25
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             542..559
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     566..585
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        566..584
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aatcggactg gttccgcgtg gatccatgga acgagctata caaggaaacg acgctaggga
       61 acaagctaac agtgaacgtt gggatggagg atcaggaggt accacttctc ccttcaaact
      121 tcctgacgaa agtccgagtt ggactgagtg gcggctacat aacgatgaga cgaattcgaa
      181 tcaagataat ccccttggtt tcaaggaaag ctggggtttc gggaaagttg tatttaagag
      241 atatctcaga tacgacagga cggaagcttc actgcacaga gtccttggat cttggacggg
      301 agattcggtt aactatgcag catctcgatt tttcggtttc gaccagatcg gatgtaccta
      361 tagtattcgg tttcgaggag ttagtatcac cgtttctgga gggtcgcgaa ctcttcagca
      421 tctctgtgag atggcaattc ggtctaagca agaactgcta cagcttgccc caatcgaagt
      481 ggaaagtaat gtatcaagag gatgccctga aggtactcaa accttcgaaa aagaaagcga
      541 gcaccaccac caccaccact gaatctaata cgactcacta taggggagct caagcattgg
      601 gac
//
