LOCUS       sequence-67500-e         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Shen MW, Fang F, Sandmeyer S, Da Silva NA
  TITLE     Development and characterization of a vector set with regulated 
            promoters for systematic metabolic engineering in Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2012 Dec;29(12):495-503. doi: 10.1002/yea.2930. Epub 2012 Nov
            19.
  PUBMED    23166051
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.2930"; 
            journalName: "Yeast"; date: "2012-12"; volume: "29"; issue: "12"; 
            pages: "495-503"
COMMENT     Sequence Label: pXP741
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    575..608
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 acaaaacggc atcagccttc ttggaggctt ccagcgcctc atctggaagt gggacacctg
       61 tagcttcgat agcagcacca ccaattaaat gattttcgaa atcgaacttg acattggaac
      121 gaacatcaga aatagcttta agaaccttaa tggcttcggc tgtgatttct tgaccaacgt
      181 ggtcacctgg caaaacgacg atcttcttag gggcagacat tagaatggta tatccttgaa
      241 atatatatat atattgctga aatgtaaaag gtaagaaaag ttagaaagta agacgattgc
      301 taaccaccta ttggaaaaac aataggtcct taaataatat tgtcaacttc aagtattgtg
      361 atgcaagcat ttagtcatga acgcttctct attctatatg aaaagccggt tccggcgctc
      421 tcacctttcc tttttctccc aatttttcag ttgaaaaagg tatatgcgtc aggcgacctc
      481 tgaaattaac aaaaaatttc cagtcatcga atttgattct gtgcgatagc gcccctgtgt
      541 gttctcgtta tgttgaggaa aaaaataatg gttaataact tcgtataatg tatgctatac
      601 gaagttatcc cgggtaccga gctcgaattc gtaacttaca cgcgcctcgt atcttttaat
      661 gatggaataa tttgggaatt tactctgtgt ttatttattt ttatgttttg tatttggatt
      721 ttagaaagta aataaagaag gtagaagagt tacggaatga agaaaaaaaa ataaacaaag
      781 gtttaaaaaa tttcaacaaa aagcgtactt tacatatata tttattagac aagaaaagca
      841 gattaaatag atatac
//
