LOCUS       sequence-67558-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Gross GG, Junge JA, Mora RJ, Kwon HB, Olson CA, Takahashi TT, Liman 
            ER, Ellis-Davies GC, McGee AW, Sabatini BL, Roberts RW, Arnold DB
  TITLE     Recombinant probes for visualizing endogenous synaptic proteins in 
            living neurons.
  JOURNAL   Neuron. 2013 Jun 19;78(6):971-85. doi: 10.1016/j.neuron.2013.04.017.
  PUBMED    23791193
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2013.04"; journalName: "Neuron"; date: 
            "2013-06-19- 19"; volume: "78"; issue: "6"; pages: "971-85"
COMMENT     Sequence Label: pCAG_GPHN.FingR-mKate2-IL2RGTC
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             439..564
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the rat zinc finger protein Kid-1 
                     (Witzgall et al., 1994)"
                     /label=KRAB-A
                     /translation="VSVTFEDVAVLFTRDEWKKLDLSQRSLYREVMLENYSNLASM"
     intron          607..739
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     722..741
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     complement(801..821)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 gccagatact gcgacctccc tagcaaactg gggcacagat ctagatttca gtgccagatt
       61 tgcatgcgca actttagccg caaaagcacc ctgaccgatc atattcgcac ccataccggc
      121 gaaaaaccgt ttgcgtgcga tatttgcggc cgcaaatttg cggcgcgcag cacccgcacc
      181 acccatacca aaattcatac cggcagccag aaaccgtttc agtgccgcat ttgcatgcgc
      241 aactttagcc gcagcgatag cctgagcaaa catattcgca cccataccgg cgaaaaaccg
      301 tttgcgtgcg atatttgcgg ccgcaaattt gcgcagcgca gcaacctgaa agtgcatacc
      361 aaaattcatc tgcgcggcag ccagctgatc gatggtgtcg acgctcctga acaacgtgaa
      421 ggtgcttctc aagtttctgt ttctgttact tttgaagatg ttgctgttct ttttactcgt
      481 gatgaatgga aaaaacttga tctttctcaa cgttctcttt atcgtgaagt tatgcttgaa
      541 aattattcta atcttgcttc tatggcttag cgtacaagta aagcggcccc ggactcgagg
      601 ccgcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa actgggcttg
      661 tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac tgacatccac
      721 tttgcctttc tctccacagg tgtcgacaat caacctctgg attacaaaat ttgtgaaaga
      781 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
      841 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
      901 tggt
//
