LOCUS       sequence-67576-e         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Dagliyan O, Shirvanyants D, Karginov AV, Ding F, Fee L, 
            Chandrasekaran SN, Freisinger CM, Smolen GA, Huttenlocher A, Hahn 
            KM, Dokholyan NV
  TITLE     Rational design of a ligand-controlled protein conformational 
            switch.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6800-4. doi: 
            10.1073/pnas.1218319110. Epub 2013 Apr 8.
  PUBMED    23569285
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1218319110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-04-23- 23"; volume: "110"; issue: "17"; pages: "6800-4"
COMMENT     Sequence Label: pUSE-Src-YF-UniRapR-mCerulean-myc
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(310..339)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(352..603)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(394..415)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     743..762
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agctggtacg agctcggatc cactagtcca gtgtggtgga attctgcaga tcatggcagg
       61 catgggacag tcactgntac cagcctccac ctgtccttcc aggctgcccc atccctgaag
      121 cccttccatg ctccaaaagg ggcattgccg aggcaacgca gccccagaga gaggatgaag
      181 ggaggcaggt gcatcggagg caacacccgg aagcctagag gtgacagttc tgcgcacaca
      241 gtccctatag gttctccccg ggctggtact gtggctctag aggtgacagt tctgcgcaca
      301 cagtcctcac aggtcctcct cggagatcag cttctgctcc ccgcttccgg acttgtacag
      361 ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc
      421 gcgcttctcg ttggggtctt tgctcagctt ggactgggtg ctcaggtagt ggttgtcggg
      481 cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac
      541 gctgccgtcc tcgatgttgt ggcggatctt gaagttggcc ttgatgccgt tcttctgctt
      601 gtcggcggtg atatagacgt tgtcgctgat ggcgttgtac tccagcttgt gccccaggat
      661 gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc
      721 ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg
      781 ctcctgg
//
