LOCUS       sequence-67586-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  TITLE     Howard Steinman lab unpublished sacB bacterial exchange vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pNPTS138 Cm
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     21..43
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     35..52
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     36..52
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(239..255)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(239..255)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(252..274)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    263..279
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(287..293,294..311,312..317))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    332..353
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(494..513)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtccgtaata
       61 cgactcactt aaggccttga ctagagggtc gacgcatgcc tgtacatccg gagacgcgtc
      121 acggccgaag ctagcgaatt cgtggatcca gatatcctgc agagaagctt ggcgccagcc
      181 ggcttcaatt gcacgggccc cactagtgag tcgtattacg tagcttggcg taatcatggt
      241 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
      301 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttacatgtga
      361 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      421 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      481 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctcttcc
      541 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      601 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      661 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      721 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      781 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      841 cggctacact agaagaacag ta
//
