LOCUS       sequence-67612-e         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Codon optimized Cas9_SV40 NLS with intron.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Friedland AE, Tzur YB, Esvelt KM, Colaiacovo MP, Church GM, Calarco 
            JA
  TITLE     Heritable genome editing in C. elegans via a CRISPR-Cas9 system.
  JOURNAL   Nat Methods. 2013 Jun 30. doi: 10.1038/nmeth.2532.
  PUBMED    23817069
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Jun 30. doi: 10.1038/nmeth.2532."
COMMENT     Sequence Label: Peft-3::cas9-SV40_NLS::tbb-2 3'UTR
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(27..350)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     CDS             complement(359..379)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atgaccatga ttacgccaag ctttgagact tttttcttgg cggcacaata aagtttcctc
       61 tttttgttgg gtcactcaac gaacacaata caaatatttg agcgctcgca tttttctcaa
      121 cctttcgggt ttaaagcact tcaaaatgga attgaagaga gattgtgaaa gtacatcaga
      181 cagaatcaca ttttgatagc attcacttca ctcagatgca aacttttcag tgactataaa
      241 aagtcataaa ataataacat taaaaaagca aataaattag cgagagaatt ttttgacaaa
      301 aagaaagaag agtgatagag aagaagggaa tgcttgaaag gattttgcat ttatctcaga
      361 ccttacgctt cttctttggg tcggcacggg agtctcctcc gagctgggag aggtcgatac
      421 gggtctcgta gagtccggtg atggactggt ggatgagggt ggcgtcgagg acctccttgg
      481 tggaggtgta acgcttacgg tcgatggtgg tgtcgaagta cttgaaggcg gctggggctc
      541 cgaggttggt gagggtgaag aggtggatga tgttctcggc ctgctcacgg attggcttgt
      601 cacggtgctt gttgtaggcg gagaggacct tgtcgaggtt ggcgtcggcg aggatgacac
      661 gcttggagaa ctcggagatc tgctcgatga tctcgtcgag gtagtgcttg tgctgctcga
      721 cgaagagctg cttctgctcg ttgtcctctg gggatccctt gagcttctcg tagtgggagg
      781 cgaggtagag gaagttgacg tacttggatg ggagggcgag ctcgtttccc ttctggagct
      841 ctccggcgga ggcgagcata cgcttacgtc cgttctcgag ctcgaagagg gagtactttg
      901 ggagcttgat gatgaggtcc ttcttg
//
