LOCUS       sequence-67616-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Codon optimized Cas9_SV40 NLS with intron.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Friedland AE, Tzur YB, Esvelt KM, Colaiacovo MP, Church GM, Calarco 
            JA
  TITLE     Heritable genome editing in C. elegans via a CRISPR-Cas9 system.
  JOURNAL   Nat Methods. 2013 Jun 30. doi: 10.1038/nmeth.2532.
  PUBMED    23817069
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Jun 30. doi: 10.1038/nmeth.2532."
COMMENT     Sequence Label: Peft-3::cas9-SV40_NLS::tbb-2 3'UTR
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(309..333)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctcgagacgg tggaagaagg agtcgtcgac cttggccatc tcgttggaga agatctcctg
       61 gaggtagcag atacggttct tacgacgggt gtaacgacga cgggcggtac gcttgagacg
      121 ggtggcctcg gcggtctctc cggagtcgaa gaggagggct ccgatgaggt tcttcttgat
      181 ggagtgacgg tcggtgtttc cgaggacctt gaacttcttg gatgggacct tgtactcgtc
      241 ggtgatgacg gcccatccga cggagttggt tccgatgtcg agtccgatgg agtacttctt
      301 gtccattaag cctgcttttt tgtacaaact tgtgagcaaa gtgtttccca actgaaaaaa
      361 aaacaattta atttaaaagt aagaagagtg cggacggtag agagaataaa agtcgaaaaa
      421 attatcctag aaacttctaa tatttttaaa aattaaaaaa atataaaaag tgaggtcaca
      481 aataagacag ttagaacatt tgttgaaagc ctaaaataaa aattcgacta aacagtgaga
      541 acaggaaaaa cgctgggact gaatgtttga cctcattata aattcataaa atttaaattt
      601 cctaatctgg ataaaaacaa agataaaaac aaaataaacc aacaaaaaac caacatgatt
      661 agtcagatga ccagaaaact cacgttaatt tcacaaggcc caaaaaaatc tctccccctc
      721 tcgttgctgc ctgcacatct aactcctagc acgaaaatgt accgtacacc atttcaaaca
      781 ctctcggcgg gagtgttgca tactttttct ctctggcagt ttattttcaa taaattcttc
      841 atttttatat tgtataatgt ctttatttta tgaaaaattc attaatttaa cagaaacaat
      901 gg
//
