LOCUS       sequence-67618-e         987 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  klp-12 targeting sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Friedland AE, Tzur YB, Esvelt KM, Colaiacovo MP, Church GM, Calarco 
            JA
  TITLE     Heritable genome editing in C. elegans via a CRISPR-Cas9 system.
  JOURNAL   Nat Methods. 2013 Jun 30. doi: 10.1038/nmeth.2532.
  PUBMED    23817069
REFERENCE   2  (bases 1 to 987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Jun 30. doi: 10.1038/nmeth.2532."
COMMENT     Sequence Label: PU6::klp-12_sgRNA
FEATURES             Location/Qualifiers
     source          1..987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    24..260
                     /label=3' CeU6
                     /note="region downstream of the C. elegans K09B11.12 U6 
                     snRNA gene"
     misc_RNA        complement(268..343)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(364..863)
                     /label=CeU6 promoter
                     /note="promoter for the C. elegans K09B11.12 U6 snRNA gene 
                     (Friedland et al., 2013)"
     primer_bind     complement(870..887)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(870..886)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(879..901)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 atgaccatga ttacgccaag cttcacagcc gactatgttt ggcgtcgaga ttttgtaatt
       61 ttctttggaa tattgtcaaa aaaaatcaat tttttttcga gtttcctaat aaaatttttt
      121 ggaccaattt aatcaactct ggcaacttta aaatcgtccc tccgggaatt tacctttaac
      181 caagattagt tgaaacattg aactcaaaaa tttagagtat ttcattttag tttcaaaaag
      241 ggatatctag aatattattg aaaaaaagca ccgactcggt gccacttttt caagttgata
      301 acggactagc cttattttaa cttgctattt ctagctctaa aacccaattg taacttgtgg
      361 atcaaacatt tagatttgca attcaattat atagggacca tttatcatgc agctgacata
      421 ttgagagtcc tgtaagacac tataccaaat ggggatgtag atcaataatt tgtgggggag
      481 gcatgtgtga ttcgtactcg ttcggataga gctttggtga atgtccaggc ttctgaaacg
      541 taggtgagcg ctggaaggac aattgagtcg aacagattcg cacgaatctt cttgtcggtg
      601 atggagtcgg tggcattctt gattccattg aatgcagccc aggctgctcg acgtcttcgg
      661 tggatttccg gcatcaagtt gttttgggcg ttgatttgac gaccgaggta gatgtactcg
      721 tcgacgtcgt cgagctgggt ggtgggggaa gggctaccga agtagacttt actggggtca
      781 gcgaatcggt ttcgcaagac tttcgtcttc ccagtattga tctcgagacc tacttcagag
      841 catttttgta cgagttcttg gaggaattca ctggccgtcg ttttacaacg tcgtgactgg
      901 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      961 cgtaatagcg aagaggcccg caccgat
//
