LOCUS       sequence-67620-e         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  unc-119 targeting sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Friedland AE, Tzur YB, Esvelt KM, Colaiacovo MP, Church GM, Calarco 
            JA
  TITLE     Heritable genome editing in C. elegans via a CRISPR-Cas9 system.
  JOURNAL   Nat Methods. 2013 Jun 30. doi: 10.1038/nmeth.2532.
  PUBMED    23817069
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Jun 30. doi: 10.1038/nmeth.2532."
COMMENT     Sequence Label: PU6::unc-119_sgRNA
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    23..259
                     /label=3' CeU6
                     /note="region downstream of the C. elegans K09B11.12 U6 
                     snRNA gene"
     misc_RNA        complement(267..342)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(363..862)
                     /label=CeU6 promoter
                     /note="promoter for the C. elegans K09B11.12 U6 snRNA gene 
                     (Friedland et al., 2013)"
     primer_bind     complement(869..886)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(869..885)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(878..900)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tgaccatgat tacgccaagc ttcacagccg actatgtttg gcgtcgagat tttgtaattt
       61 tctttggaat attgtcaaaa aaaatcaatt ttttttcgag tttcctaata aaattttttg
      121 gaccaattta atcaactctg gcaactttaa aatcgtccct ccgggaattt acctttaacc
      181 aagattagtt gaaacattga actcaaaaat ttagagtatt tcattttagt ttcaaaaagg
      241 gatatctaga atattattga aaaaaagcac cgactcggtg ccactttttc aagttgataa
      301 cggactagcc ttattttaac ttgctatttc tagctctaaa actctttaat ttcagaaaat
      361 tcaaacattt agatttgcaa ttcaattata tagggaccat ttatcatgca gctgacatat
      421 tgagagtcct gtaagacact ataccaaatg gggatgtaga tcaataattt gtgggggagg
      481 catgtgtgat tcgtactcgt tcggatagag ctttggtgaa tgtccaggct tctgaaacgt
      541 aggtgagcgc tggaaggaca attgagtcga acagattcgc acgaatcttc ttgtcggtga
      601 tggagtcggt ggcattcttg attccattga atgcagccca ggctgctcga cgtcttcggt
      661 ggatttccgg catcaagttg ttttgggcgt tgatttgacg accgaggtag atgtactcgt
      721 cgacgtcgtc gagctgggtg gtgggggaag ggctaccgaa gtagacttta ctggggtcag
      781 cgaatcggtt tcgcaagact ttcgtcttcc cagtattgat ctcgagacct acttcagagc
      841 atttttgtac gagttcttgg aggaattcac tggccgtcgt tttacaacgt cgtgactggg
      901 aaaaccc
//
