LOCUS       sequence-67621-e         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expression of human codon-optimized Cas9 nuclease and GFP, 
            plasmid optimized for expression in human pluripotent stem cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Ding Q, Regan SN, Xia Y, Oostrom LA, Cowan CA, Musunuru K
  TITLE     Enhanced efficiency of human pluripotent stem cell genome editing 
            through replacing TALENs with CRISPRs.
  JOURNAL   Cell Stem Cell. 2013 Apr 4;12(4):393-4. doi: 
            10.1016/j.stem.2013.03.006.
  PUBMED    23561441
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.03"; journalName: "Cell Stem Cell"; date: 
            "2013-04-4- 4"; volume: "12"; issue: "4"; pages: "393-4"
COMMENT     Sequence Label: pCas9_GFP
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      103..112
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg
       61 gttattgtgc tgtctcatca ttttggcaaa gaattcgcgg ccgccaccat ggacaagaag
      121 tactccattg ggctcgatat cggcacaaac agcgtcggct gggccgtcat tacggacgag
      181 tacaaggtgc cgagcaaaaa attcaaagtt ctgggcaata ccgatcgcca cagcataaag
      241 aagaacctca ttggcgccct cctgttcgac tccggggaga cggccgaagc cacgcggctc
      301 aaaagaacag cacggcgcag atatacccgc agaaagaatc ggatctgcta cctgcaggag
      361 atctttagta atgagatggc taaggtggat gactctttct tccataggct ggaggagtcc
      421 tttttggtgg aggaggataa aaagcacgag cgccacccaa tctttggcaa tatcgtggac
      481 gaggtggcgt accatgaaaa gtacccaacc atatatcatc tgaggaagaa gcttgtagac
      541 agtactgata aggctgactt gcggttgatc tatctcgcgc tggcgcatat gatcaaattt
      601 cggggacact tcctcatcga gggggacctg aacccagaca acagcgatgt cgacaaactc
      661 tttatccaac tggttcagac ttacaatcag cttttcgaag agaacccgat caacgcatcc
      721 ggagttgacg ccaaagcaat cctgagcgct aggctgtcca aatcccggcg gctcgaaaac
      781 ctcatcgcac agctccctgg ggagaagaag aacggcctgt ttggtaatct tatcgccctg
      841 tcactcgggc tgacccccaa ctttaaatct aacttcgacc tggccgaaga tgccaagctt
      901 caactgagca aagacaccta cgatgatgat ctcgacaatc tgctggccc
//
