LOCUS       sequence-67623-e        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for Ca2+ inside primary cilia.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Su S, Phua SC, Derose R, Chiba S, Narita K, Kalugin PN, Katada T, 
            Kontani K, Takeda S, Inoue T
  TITLE     Genetically encoded calcium indicator illuminates calcium dynamics 
            in primary cilia.
  JOURNAL   Nat Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647.
  PUBMED    24056873
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647."
COMMENT     Sequence Label: 5HT6-G-GECO1.0
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      102..111
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag cttggtaccg agctcggatc cgccaccatg gttccagagc
      121 ccggccctgt caacagtagc accccagcct ggggccccgg gccaccgcca gccccagggg
      181 gcagcggctg ggtggccgct gcgctgtgcg tggtcatcgt gctgacggca gccgccaatt
      241 cgctgctgat cgcgctcatc tgcacgcagc ccgcgctgcg caacacgtct aacttcttcc
      301 tggtgtcgct cttcacgtcg gacctgatgg tgggattggt ggtgatgccc ccagccatgc
      361 tgaacgcgct gtatgggcgc tgggtactag ctcgcggcct ctgtctgctt tggaccgcct
      421 tcgacgtgat gtgctgcagc gcctccattc tcaacctctg cctcatcagc ctggaccgct
      481 acctgctcat cctctcgccg ctgcgctaca agctgcgcat gacagccccg cgtgccctgg
      541 cgctcatcct gggtgcctgg agcctcgccg cgctggcctc cttcctgccc ctcttgctgg
      601 gctggcatga actgggcaaa gctcgaacat ctgccccggg ccagtgccgc ctattggcca
      661 gcctgcctta tgtcctcgtg gcgtccggcg tcaccttttt cctgccttcg ggtgccatct
      721 gcttcaccta ctgcaggatc ttgctggctg cccgcaagca ggcggtgcaa gtggcctcgc
      781 tcaccacggg cacggctacg gctggccagg ccttggaaac cttgcaggtg cccaggacac
      841 cacgcccagg gatggagtct gctgacagta ggcgtctgac caccaagcat agcaggaagg
      901 ccttgaaggc cagcctgacc ctcggcatcc tgctgagcat gttctttgtc acctggctgc
      961 ccttctttgt ggccagcata gctcaggccg tatgtgactg catctctcca ggtc
//
