LOCUS       sequence-67624-e        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for Ca2+ inside primary cilia with mCherry 
            reference.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Su S, Phua SC, Derose R, Chiba S, Narita K, Kalugin PN, Katada T, 
            Kontani K, Takeda S, Inoue T
  TITLE     Genetically encoded calcium indicator illuminates calcium dynamics 
            in primary cilia.
  JOURNAL   Nat Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647.
  PUBMED    24056873
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647."
COMMENT     Sequence Label: 5HT6-mCherry-G-GECO1.0
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             57..113
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     326..347
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(714..733)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gnggcntggn cgagctgtac aagtccggac tcagatccac catggtcgac tcatcacgtc
       61 gtaagtggaa taagacaggt cacgcagtca gagctatagg tcggctgagc tcactcgaga
      121 acgtctatat caaggccgac aagcagaaga acggcatcaa ggcgaacttc aagatccgcc
      181 acaacatcga ggacggcggc gtgcagctcg cctaccacta ccagcagaac acccccatcg
      241 gcgacggccc cgtgctgctg cccgacaacc actacctgag cgtgcagtcc atactttcga
      301 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
      361 tcactctcgg catggacgag ctgtacaagg gcggtaccgg agggagcatg gtgagcaagg
      421 gcgaggagct gttcaccggg gtggtgccca tccaggtcga gctggacggc gacgtaaacg
      481 gccacaagtt cagcgtgtcc ggcgagggtg agggcgatgc cacctacggc aagctgaccc
      541 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
      601 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
      661 tcaagtccgc catgcccgaa ggctacatcc aggagcgcac catcttcttc aaggacgacg
      721 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
      781 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
      841 acacgcgtga ccaactgact gaagagcaga tcgcagaatt taaagaggct ttctccctat
      901 ttgacaagga cggggatggg acgataacaa ccaaggagct ggggacggtg atgcggtctc
      961 tggggcagaa ccccacagaa gcagagctgc aggacatgat caatgaagta gatgccgacg
//
