LOCUS       sequence-67636-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  APEX targeted to the mitochondrial matrix of mammalian cells. Used 
            for proteomic mapping of mitochondria. This construct has been 
            replaced by APEX2 (construct 49386). .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Rhee HW, Zou P, Udeshi ND, Martell JD, Mootha VK, Carr SA, Ting AY
  TITLE     Proteomic Mapping of Mitochondria in Living Cells via Spatially 
            Restricted Enzymatic Tagging.
  JOURNAL   Science. 2013 Jan 31.
  PUBMED    23371551
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2013 Jan 31."
COMMENT     Sequence Label: pcDNA3-mito-APEX
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             186..227
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttgcggccgc caccatgctg gccacccgcg
      121 tgttcagcct ggtgggcaag cgcgccatca gcaccagcgt gtgcgtgcgc gcccacaagg
      181 atccaggcaa gcccatcccc aaccccctgc tgggcctgga cagcaccgga aagtcttacc
      241 caactgtgag tgctgattac caggacgccg ttgagaaggc gaagaagaag ctcagaggct
      301 tcatcgctga gaagagatgc gctcctctaa tgctccgttt ggcattccac tctgctggaa
      361 cctttgacaa gggcacgaag accggtggac ccttcggaac catcaagcac cctgccgaac
      421 tggctcacag cgctaacaac ggtcttgaca tcgctgttag gcttttggag ccactcaagg
      481 cggagttccc tattttgagc tacgccgatt tctaccagtt ggctggcgtt gttgccgttg
      541 aggtcacggg tggacctaag gttccattcc accctggaag agaggacaag cctgagccac
      601 caccagaggg tcgcttgccc gatgccacta agggttctga ccatttgaga gatgtgtttg
      661 gcaaagctat ggggcttact gaccaagata tcgttgctct atctgggggt cacactattg
      721 gagctgcaca caaggagcgt tctggatttg agggtccctg gacctctaat cctcttattt
      781 tcgacaactc atacttcacg gagttgttga gtggtgagaa ggaaggtctc cttcagctac
      841 cttctgacaa ggctcttttg tctgaccctg tattccgccc tctcgttgat aaatatgca
//
