LOCUS       sequence-67639-e         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid is used to reconstitute the complete Type II CRISPR 
            system from S. pyogenes and contains the host factor RNaseIII and 
            tracrRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, 
            Jiang W, Marraffini LA, Zhang F
  TITLE     Multiplex Genome Engineering Using CRISPR/Cas Systems.
  JOURNAL   Science. 2013 Jan 3.
  PUBMED    23287718
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2013 Jan 3."
COMMENT     Sequence Label: pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(866..884)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 tttcaggtgt cgtgacgtac cgccaccatg aagcagctgg aggagttact ttctacctct
       61 ttcgacatcc agtttaatga cctgaccctg ctggaaaccg ccttcactca cacctcctac
      121 gcgaatgagc accgcctact gaatgtgagc cacaacgagc gcctggagtt tctgggggat
      181 gctgtcttac agctgatcat ctctgaatat ctgtttgcca aataccctaa gaaaaccgaa
      241 ggggacatgt caaagctgcg ctccatgata gtcagggaag agagcctggc gggctttagt
      301 cgtttttgct cattcgacgc ttatatcaag ctgggaaaag gcgaagagaa gtccggcggc
      361 aggaggcgcg atacaattct gggcgatctc tttgaagcgt ttctgggcgc acttctactg
      421 gacaaaggga tcgacgcagt ccgccgcttt ctgaaacaag tgatgatccc tcaggtcgaa
      481 aagggaaact tcgagagagt gaaggactat aaaacatgtt tgcaggaatt tctccagacc
      541 aagggagatg tagcaataga ttatcaggta ataagtgaga aaggaccagc tcacgccaaa
      601 caattcgaag ttagcatcgt tgttaatggc gcagtgttgt cgaagggctt gggtaaatca
      661 aaaaaactgg ccgagcagga cgctgctaaa aacgccctcg ctcagctcag cgaggtagga
      721 tccgtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
      781 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
      841 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
      901 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
      961 c
//
