LOCUS       sequence-67640-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of green fluorescent genetically encoded Ca2+ 
            indicator for optical imaging (version 1.0).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Zhao Y, Araki S, Wu J, Teramoto T, Chang YF, Nakano M, Abdelfattah 
            AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE
  TITLE     An Expanded Palette of Genetically Encoded Ca2+ Indicators.
  JOURNAL   Science. 2011 Sep 30;333(6051):1888-91. doi: 10.1126/science.1208592
  PUBMED    21903779
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2011-09-30- 30"; volume: "333"; 
            issue: "6051"; pages: "1888-91"
COMMENT     Sequence Label: CMV-G-GECO1.0
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(58..501)
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYIGAAEL
                     RHVMTNLGEKLTDEEVDEMIRVADIDGDGQVNYEEFVQMMTAK"
     primer_bind     617..636
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gggccctcta gatgcatgct cgagcggccg ccagtgtgat ggatatctgc agaattctta
       61 cttcgctgtc atcatttgta caaactcttc gtagtttacc tgaccatccc catcgatgtc
      121 tgctaccctg atcatttcat caacctcttc atctgttaac ttctctccaa ggtttgtcat
      181 cacgtggcga agctctgctg cgccgatgta gccattgccg tccttatcaa acacacggaa
      241 cgcttctcta atttcttctt cactgtctgt gtctttcatt tttcttgcca tcattgtcag
      301 gaactcaggg aagtcgattg tgccgtcacc gtcggcatct acttcattga tcatgtcctg
      361 cagctctgct tctgtggggt tctgccccag agaccgcatc accgtcccca gctccttggt
      421 tgttatcgtc ccatccccgt ccttgtcaaa tagggagaaa gcctctttaa attctgcgat
      481 ctgctcttca gtcagttggt cacgcgtgtt gtactccagc ttgtgcccca ggatgttgcc
      541 gtcctccttg aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa
      601 cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg
      661 gatgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta
      721 gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg agggtgggcc agggcacggg
      781 cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccgta
//
