LOCUS       sequence-67645-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Shukla AK, Kim J, Ahn S, Xiao K, Shenoy SK, Liedtke W, Lefkowitz RJ
  TITLE     Arresting a transient receptor potential (TRP) channel: 
            beta-arrestin 1 mediates ubiquitination and functional 
            down-regulation of TRPV4.
  JOURNAL   J Biol Chem. 2010 Sep 24;285(39):30115-25. doi: 
            10.1074/jbc.M110.141549. Epub 2010 Jul 22.
  PUBMED    20650893
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M110.141549"; journalName: "J Biol Chem"; date: 
            "2010-09-24- 24"; volume: "285"; issue: "39"; pages: "30115-25"
COMMENT     Sequence Label: pcDNA3.0 FLAG TRPV4
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             106..129
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    196..330
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(220..239)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     274..293
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(447..467)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(447..465)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(486..502)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(486..502)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(499..521)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    510..526
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(534..540,541..558,559..564))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    579..600
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(717..734)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggcacagatg aagtggtggt ccccctggat aacctaggga accccaactg tgacggccac
       61 cagcaaggtt atgctcccaa gtggagggcg gaggacgcac cactggatta caaggacgat
      121 gacgataagg tgactataaa gacgaagacg ataaatgact cgagcctcta gaactatagt
      181 gagtcgtatt acgtagatcc agacatgata agatacattg atgagtttgg acaaaccaca
      241 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
      301 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
      361 caggttcagg gggaggtgtg ggaggttttt taattcgcgg cgcgccgcgg cgccaatgca
      421 ttgggcccgg tacccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
      481 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
      541 cgagccggga gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
      601 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
      661 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
      721 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
      781 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
      841 g
//
