LOCUS       sequence-67646-e         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Carlson LA, Hurley JH
  TITLE     In vitro reconstitution of the ordered assembly of the endosomal 
            sorting complex required for transport at membrane-bound HIV-1 Gag 
            clusters.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Oct 16;109(42):16928-33. doi: 
            10.1073/pnas.1211759109. Epub 2012 Oct 1.
  PUBMED    23027949
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1211759109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-10-16- 16"; volume: "109"; issue: "42"; pages: 
            "16928-33"
COMMENT     Sequence Label: pGagMBPHis
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(37..54)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(79..102)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     regulatory      210..219
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agcttccttt cgggctttgt tagcagccgg atctcagtgg tggtggtggt ggtgctcgag
       61 tgacgagctc gaattagtct gcgcgtcttt cagggcttca tcgacagtct gacgaccgct
      121 ggcggcgttg atcaccgcag tacgcacggc ataccagaaa gcggacatct gcgggatgtt
      181 cggcatgatt tcacctttct gggcgttttc catggtggcg gcaatacgtg gatctttcgc
      241 caactcttcc tcgtaagact tcagcgctac ggcacccagc ggtttgtctt tattaaccgc
      301 ttccagacct tcatcagtca gcagatagtt ttcgaggaac tcttttgcca gctctttgtt
      361 cggactggcg gcgttaatac ctgcgctcag cacgccaacg aacggtttgg atggttgacc
      421 cttgaaggtc ggcagtaccg ttacaccata attcactttg ctggtgtcga tgttggacca
      481 tgcccacggg ccgttgatgg tcatcgctgt ttcgccttta ttaaaggcag cttctgcgat
      541 ggagtaatcg gtgtctgcat tcatgtgttt gtttttaatc aggtcaacca ggaaggtcag
      601 acccgctttc gcgccagcgt tatccacgcc cacgtcttta atgtcgtact tgccgttttc
      661 atacttgaac gcataacccc cgtcagcagc aatcagcggc caggtgaagt acggttcttg
      721 caggttgaac atcagcgcgc tcttaccttt cgctttcagt tctttatcca gcgccgggat
      781 ctcttcccag gtttttggcg ggttcggcag cagatctttg ttataaatca gcgataacgc
      841 ttcaacagcg atcgggtaag caatcagctt gccgttgtaa cgtacggcat cccaggtaaa
      901 cggatacagc ttgtcctgga acgctttgtc cggggtgatt tcagccaaca ggcca
//
