LOCUS       sequence-67649-e         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Baskaran S, Ragusa MJ, Boura E, Hurley JH
  TITLE     Two-site recognition of phosphatidylinositol 3-phosphate by PROPPINs
            in autophagy.
  JOURNAL   Mol Cell. 2012 Aug 10;47(3):339-48. doi: 
            10.1016/j.molcel.2012.05.027. Epub 2012 Jun 14.
  PUBMED    22704557
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2012.05.027"; journalName: "Mol Cell"; date: 
            "2012-08-10- 10"; volume: "47"; issue: "3"; pages: "339-48"
COMMENT     Sequence Label: pHIS-Hsv2
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             62..79
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             101..121
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgtcgtacta
       61 ccatcaccat caccatcacg attacgatat cccaacgacc gaaaacctgt attttcaggg
      121 cgccatggga tccggaattc aaatggatgt tcgtcgacct ataagggagg cagtcaacaa
      181 caggaggaaa ccaaaatttt tgagcgtttc gtttaaccaa gatgattcgt gtttcagcgt
      241 ggcgttagag aatggatttc gtattttcaa tacagatcca ttgactagta agctatcgaa
      301 aacatttaaa gagtctgcga ccaaccaatc caggggcact gggattggct ataccaggat
      361 gctttatcgt acgaattaca tcgcactagt cggaggtggt aaacgaccaa ggcatgctct
      421 aaataaactg atcatctggg atgatctttt gcaaaaggaa acgattactt tgaagtttat
      481 gtcttccatt aaagacgtgt ttttatctag gattcatatt gtggtagtcc tggagaacac
      541 aatagagatc ttccaatttc aaaccaatcc tcaaagaatt tgtcctattt tggatatccc
      601 tcccaatgga tcagtggact atgtcgtttg tagcagcaaa catctccagt cgcaagcatc
      661 gcagtcacag tctaaaatct tagaaatcat tgcattccca tcgaataaat gcgtaggcca
      721 aattcaagta gccgacctat cacaaataaa atataattca caaaacccga aggaatcagc
      781 gcttttgccc acttccatca ttaaagcaca taaaaatccc atcaaactgg ttagactgaa
      841 ccgtcaaggc accatggtag caacatgttc cgtccagggt acacttataa gaatcttcag
      901 tacgcataac ggtactttaa tcaaagaatt tagaagaggg gtggaca
//
