LOCUS       sequence-67650-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of green fluorescent genetically encoded Ca2+ 
            indicator for optical imaging (version 1.0).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Zhao Y, Araki S, Wu J, Teramoto T, Chang YF, Nakano M, Abdelfattah 
            AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE
  TITLE     An Expanded Palette of Genetically Encoded Ca2+ Indicators.
  JOURNAL   Science. 2011 Sep 30;333(6051):1888-91. doi: 10.1126/science.1208592
  PUBMED    21903779
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2011-09-30- 30"; volume: "333"; 
            issue: "6051"; pages: "1888-91"
COMMENT     Sequence Label: CMV-G-GECO1.0
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             114..170
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     383..404
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(771..790)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagcttggt accgagctcg gatccaccat ggtcgactca tcacgtcgta
      121 agtggaataa gacaggtcac gcagtcagag ctataggtcg gctgagctca ctcgagaacg
      181 tctatatcaa ggccgacaag cagaagaacg gcatcaaggc gaacttcaag atccgccaca
      241 acatcgagga cggcggcgtg cagctcgcct accactacca gcagaacacc cccatcggcg
      301 acggccccgt gctgctgccc gacaaccact acctgagcgt gcagtccata ctttcgaaag
      361 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
      421 ctctcggcat ggacgagctg tacaagggcg gtaccggagg gagcatggtg agcaagggcg
      481 aggagctgtt caccggggtg gtgcccatcc aggtcgagct ggacggcgac gtaaacggcc
      541 acaagttcag cgtgtccggc gagggtgagg gcgatgccac ctacggcaag ctgaccctga
      601 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
      661 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
      721 agtccgccat gcccgaaggc tacatccagg agcgcaccat cttcttcaag gacgacggca
      781 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatc
//
