LOCUS       sequence-67658-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Ren X, Hurley JH
  TITLE     VHS domains of ESCRT-0 cooperate in high-avidity binding to 
            polyubiquitinated cargo.
  JOURNAL   EMBO J. 2010 Mar 17;29(6):1045-54. Epub 2010 Feb 11.
  PUBMED    20150893
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2010-03-17"; volume: "29(6)"; pages: "1045-54. Epub 2010 Feb 
            11"
COMMENT     Sequence Label: pGST1-GGA2-VHS
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..45
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     regulatory      47..56
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(620..642)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(787..805)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 catcctccaa aaatcgatac gaccgaaaac ctgtattttc agggcgccat ggcggcgacc
       61 gcggtggcgg cggctgtggc gggaaccgag tcggcccagg gtcccccggg cccgacagcg
      121 tcgctggagc tgtggctgaa caaagccaca gacccaagca tgtcggaaca ggattggtca
      181 gctatccaga atttctgtga gcaggtgaac actgacccca atggccccac acatgcgccc
      241 tggctactgg cccacaagat ccagtctccg caagagaagg aagctcttta tgccttaacg
      301 gtgctggaga tgtgcatgaa ccactgtggg gagaagttcc acagcgaggt ggccaaattt
      361 cgtttcctga acgaactgat caaagtgttg tccccaaagt acctggggtc ctgggccaca
      421 ggaaaagtta aaggaagagt cattgaaata ctcttcagtt ggacagtctg gtttccggaa
      481 gacatcaaga ttcgagacgc ttatcagatg ctgaagaaac aaggaattat aaaacaagac
      541 taagagcctg cagtctcgag cggccgcatc gtgactgact gacgatctgc ctcgcgcgtt
      601 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
      661 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
      721 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtataat tcttgaagac
      781 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
      841 agacgtcagg tggcacttt
//
