LOCUS       sequence-67665-e         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Ragusa MJ, Stanley RE, Hurley JH
  TITLE     Architecture of the Atg17 complex as a scaffold for autophagosome 
            biogenesis.
  JOURNAL   Cell. 2012 Dec 21;151(7):1501-12. doi: 10.1016/j.cell.2012.11.028. 
            Epub 2012 Dec 6.
  PUBMED    23219485
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.11.028"; journalName: "Cell"; date: 
            "2012-12-21- 21"; volume: "151"; issue: "7"; pages: "1501-12"
COMMENT     Sequence Label: Atg17-Atg31-Atg29-pST39 (Auto 16)
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(373..393)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(415..432)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             452..474
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 atttaataga ttatcatttt gtaaagtagg ggtatattta tgtattaaat cagacatccc
       61 ttcattcact tgatcatttg aatacttctc aagatctctt tttttctcca attgtagttg
      121 taaaagctct aagtgttcag caaataattt atatgttctc tttttaagaa aaaattcagg
      181 cgtttcaaat atttttgata aattctgcca atctatttga tcttgtgaat aattcaaatt
      241 tgataccatt gtccaaagct gtcgctcctt tgttccattc cactcaaatt tgggaggatc
      301 caagaatccc tgtggtctcc tacccttaac tttgatatat acaactgtgt ttgtactatt
      361 cataatcatg gcgccctgaa aatacaggtt ttcggtcgtt gggatatcgt aatcgtgatg
      421 gtgatggtga tggtagtacg acatatgtat atctccttct taaagttaaa caaaattatt
      481 ggtacctcat acggaattgg agagcatttg taattgttca ttttgtgtga cgtagagttt
      541 aattaagtca ttcaaactca aatcgcgaag aaaaggagac agatcgcaaa attgtgaaat
      601 tacatcgagc tcaatgtccg agtccactct gaatctatca ctgtcaacgc taagttcttc
      661 ctcttcatca ttagatttat catcaccatg tgatgataat gttctcattt ggttttcctc
      721 caagctgaga ctatttgtcc tatgtgacaa aagctcatat tggtcgctga ttagtgtgat
      781 atcctcaaga gatcccga
//
