LOCUS       sequence-67669-e         564 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of WT c-Jun.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Aguilera C, Nakagawa K, Sancho R, Chakraborty A, Hendrich B, Behrens
            A
  TITLE     c-Jun N-terminal phosphorylation antagonises recruitment of the 
            Mbd3/NuRD repressor complex.
  JOURNAL   Nature. 2011 Jan 13;469(7329):231-5. doi: 10.1038/nature09607. Epub 
            2011 Jan 2.
  PUBMED    21196933
REFERENCE   2  (bases 1 to 564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature09607"; journalName: "Nature"; date: "2011-01-13- 
            13"; volume: "469"; issue: "7329"; pages: "231-5"
COMMENT     Sequence Label: Flag-JunWT-Myc
FEATURES             Location/Qualifiers
     source          1..564
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        2..46
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     promoter        92..110
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      145..154
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             154..177
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg
       61 tcagatccgc tagcgattac gccaagctcg aaattaaccc tcactaaagg gaacaaaagc
      121 tggagctcca ccgcggtggc ggccgccacc atggattaca aggatgacga cgataaggcc
      181 cgggcggatc ccccgggctg caggaattcg atgactgcaa agatggaaac gaccttctac
      241 gacgatgccc tcaacgcctc gttcctccag tccgagagcg gtgcctacgg ctacagtaac
      301 cctaagatcc taaaacagag catgaccttg aacctggccg acccggtggg cagtctgaag
      361 ccgcacctcc gcgccaagaa ctcggacctt ctcacgtcgc ccgacgtcgg gctgctcaag
      421 ctggcgtcgc cggagctgga gcgcctgatc atccagtcca gcaatgggca catcaccact
      481 acaccgaccc ccacccagtt cttgtgcccc aagaacgtga ccgacgagca ggagggcttc
      541 gccgagggct tcgtgcgcgc cctg
//
