LOCUS       sequence-67683-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Koreishi M, Yu S, Oda M, Honjo Y, Satoh A
  TITLE     CK2 phosphorylates Sec31 and regulates ER-To-Golgi trafficking.
  JOURNAL   PLoS One. 2013;8(1):e54382. doi: 10.1371/journal.pone.0054382. Epub 
            2013 Jan 18.
  PUBMED    23349870
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013"; volume: "8(1):e54382. doi"; pages: " 
            10.1371/journal.pone.0054382. Epub 2013 Jan 18"
COMMENT     Sequence Label: FLAG-Sec31a S527/799/1165A #31#907
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(421..439)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(422..439)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(459..476)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    465..689
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(822..841)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttgccaacaa aaaaaattac caagaaacct attccagatg agcacctcat tctaaagacc
       61 acatttgagg atcttattca gcgctgcctt tcttcagcaa cagaccctca aaccaagagg
      121 aagctagatg atgccagcaa acgtttggag tttctgtatg ataaacttag ggaacagaca
      181 ctttcaccag caatcaccag tggtttacac aacattgcaa ggagcattga aactcgaaac
      241 tactcagaag gattgaccat gcatacccac atagttagca ccagcaactt cagtgagacc
      301 tctgctttca tgccagttct caaagttgtt ctcacccagg ccaataagct gggtgtctaa
      361 gaattctgca gatatccatc acactggcgg ccgctcgagc atgcatctag agggccctat
      421 tctatagtgt cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc cttctagttg
      481 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
      541 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
      601 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
      661 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
      721 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
      781 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
      841 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
      901 agggttccga
//
