LOCUS       sequence-67684-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Koreishi M, Yu S, Oda M, Honjo Y, Satoh A
  TITLE     CK2 phosphorylates Sec31 and regulates ER-To-Golgi trafficking.
  JOURNAL   PLoS One. 2013;8(1):e54382. doi: 10.1371/journal.pone.0054382. Epub 
            2013 Jan 18.
  PUBMED    23349870
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013"; volume: "8(1):e54382. doi"; pages: " 
            10.1371/journal.pone.0054382. Epub 2013 Jan 18"
COMMENT     Sequence Label: GFP-Sec31a 4SA #33#638
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(430..448)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(431..448)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(466..482)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(466..482)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(479..501)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    490..506
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(514..520,521..538,539..544))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    559..580
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(697..714)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttgccaacaa aaaaattacc aagaaaccta ttccagatga gcacctcatt ctaaagacca
       61 catttgagga tcttattcag cgctgccttt cttcagcaac agaccctcaa accaagagga
      121 agctagatga tgccagcaaa cgtttggagt ttctgtatga taaacttagg gaacagacac
      181 ttgcaccagc aatcaccagt ggtttacaca acattgcaag gagcattgaa actcgaaact
      241 actcagaagg attgaccatg catacccaca tagttagcac cagcaacttc agtgagacct
      301 ctgctttcat gccagttctc aaagttgttc tcacccaggc caataagctg ggtgtctaag
      361 aattcgcggc cgcctgcagg tcgaccatat gggagagctc ccaacgcgtt ggatgcatag
      421 cttgagtatt ctatagtgtc acctaaatag cttggcgtaa tcatggtcat agctgtttcc
      481 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
      541 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
      601 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
      661 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
      721 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
      781 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
      841 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
      901 caaaaatcga cgctcaagtc aga
//
