LOCUS       sequence-67687-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Hocine S, Raymond P, Zenklusen D, Chao JA, Singer RH
  TITLE     Single-molecule analysis of gene expression using two-color RNA 
            labeling in live yeast.
  JOURNAL   Nat Methods. 2013 Feb;10(2):119-21. doi: 10.1038/nmeth.2305. Epub 
            2012 Dec 23.
  PUBMED    23263691
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2305"; journalName: "Nat Methods"; date: "2013-02"; 
            volume: "10"; issue: "2"; pages: "119-21"
COMMENT     Sequence Label: pDZ274 (pLEU MET25pro MCP-2x-yeGFP)
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(4..21)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(28..744)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(31..744)
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 tcaatggtga tggtgatgat gaccggttta tttgtacaat tcatccatac catgggtaat
       61 accagcagca gtaacaaatt ctaacaagac catgtggtct ctcttttcgt ttggatcttt
      121 ggataaggca gattgagtgg ataagtaatg gttgtctggt aacaagactg gaccatcacc
      181 aattggagta ttttgttgat aatggtcagc taattgaaca gaaccatctt caatgttgtg
      241 tctaattttg aagttaactt tgataccatt cttttgtttg tcagccatga tgtaaacatt
      301 gtgagagtta tagttgtatt ccaatttgtg acctaaaatg ttaccatctt ctttaaaatc
      361 aatacctttt aattcgattc tattaactaa ggtatcacct tcaaacttga cttcagctct
      421 ggtcttgtag ttaccgtcat ctttgaaaaa aatagttctt tcttgaacat aaccttctgg
      481 catggcagac ttgaaaaagt catgttgttt catatgatct gggtatctag aaaaacattg
      541 aacaccataa gttaaagtag tgactaaggt tggccatgga actggcaatt taccagtagt
      601 acaaataaat tttaaggtca atttaccgta agtagcatca ccttcacctt caccggagac
      661 agaaaatttg tgaccattaa catcaccatc taattcaacc aaaattggga caacaccagt
      721 gaataattct tcacctttag acatgtcgac ggtatcgata agcttgatat cgaattcctg
      781 cagcccgggg gatcttttgt acaattcatc cataccatgg gtaataccag cagcagtaac
      841 aaattctaac aagaccatgt g
//
