LOCUS       sequence-67689-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Hocine S, Raymond P, Zenklusen D, Chao JA, Singer RH
  TITLE     Single-molecule analysis of gene expression using two-color RNA 
            labeling in live yeast.
  JOURNAL   Nat Methods. 2013 Feb;10(2):119-21. doi: 10.1038/nmeth.2305. Epub 
            2012 Dec 23.
  PUBMED    23263691
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2305"; journalName: "Nat Methods"; date: "2013-02"; 
            volume: "10"; issue: "2"; pages: "119-21"
COMMENT     Sequence Label: pDZ274 (pLEU MET25pro MCP-2x-yeGFP)
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        23..372
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /label=MET17 promoter
                     /note="expression is repressed by methionine"
     CDS             408..794
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2
                     /note="binds to a specific stem-loop structure in the viral
                     RNA (Peabody, 1993)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQNRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
ORIGIN
        1 ggttcgaatc ccttagctct cattattttt tgctttttct cttgaggtca catgatcgca
       61 aaatggcaaa tggcacgtga agctgtcgat attggggaac tgtggtggtt ggcaaatgac
      121 taattaagtt agtcaaggcg ccatcctcat gaaaactgtg taacataata accgaagtgt
      181 cgaaaaggtg gcaccttgtc caattgaaca cgctcgatga aaaaaataag atatatataa
      241 ggttaagtaa agcgtctgtt agaaaggaag tttttccttt ttcttgctct cttgtctttt
      301 catctactat ttccttcgtg taatacaggg tcgtcagata catagataca attctattac
      361 ccccatccat actctagata gagccctcaa ccggagtttg aagcatggct tctaacttta
      421 ctcagttcgt tctcgtcgac aatggcggaa ctggcgacgt gactgtcgcc ccaagcaact
      481 tcgctaacgg ggtcgctgaa tggatcagct ctaactcgcg ttcacaggct tacaaagtaa
      541 cctgtagcgt tcgtcagagc tctgcgcaga atcgcaaata caccatcaaa gtcgaggtgc
      601 ctaaagtggc aacccagact gttggtggtg tagagcttcc tgtagccgca tggcgttcgt
      661 acctaaatat ggaactaacc attccaattt tcgccacgaa ttccgactgc gagcttattg
      721 ttaaggcaat gcaaggtctc ctaaaagatg gaaacccgat tccctcagca atcgcagcaa
      781 actccggcat ctacggatcc cccgggctgc aggaattcga tatcaagctt atcgataccg
      841 tcgacatgtc taaa
//
